临床与实验病理学杂志2018，Vol.34Issue(1)：32-36,5.DOI:10.13315/j.cnki.cjcep.2018.01.008
MCP-1及其受体在wAMD小鼠模型视网膜中的表达及作用机制
Expression and function of MCP-1 and its receptor in wAMD model mouse
摘要
Abstract
Purpose To detect the expression and the function of MCP-1 and its receptor CCR2 in wet agerelated macular degenerative (wAMD) model mouse retina. Methods C57BL/6J mouse were enrolled into the study. Model mouse of wAMD was induced with laser. Frozen sections were prepared for histopathological tests. Immunofluorescence study for MCP-1 and CCR2 was carried out. Co-expression study for CCR2/ CDllb or CCR2/CD68 was carried out. Total protein and total mRNA from the eyes of both wAMD and wild type mouse were extracted. The expression of mRNA and protein of MCP-1 and CCR2 in the eyes were determined by reverse transcription-poly-merase chain reaction (RT-PCR) and Western blots test, respectively. Results In wild type mouse, both MCP-1 and its receptor CCR2 were not detected in the retina. However in wAMD mouse, an obvious up-regulated MCP-1 and CCR2 expression was seen in the retinal pigment epithelium (RPE) cells accompanied with the increased expression of their mRNA and protein. The co-expression study showed that CCR2 co-ex-pressed with CDllb, but not with CD68. Conclusion MCP-1 and its receptor CCR2 may play a role in the wAMD through stimulation of microglia.关键词
年龄相关性黄斑变性/MCP-1/CCR2/CNV/病理学/临床Key words
age-related macular degeneration/MCP-1/CCR2/CNV/pathology/clinical分类
医药卫生引用本文复制引用
周全,肖琳,徐景美,徐冰,Karen Wu,刘晶,卢艳,褚利群..MCP-1及其受体在wAMD小鼠模型视网膜中的表达及作用机制[J].临床与实验病理学杂志,2018,34(1):32-36,5.基金项目
北京市卫生系统高层次卫生技术人才(2014-3-049) （2014-3-049）
