临床与实验病理学杂志2018，Vol.34Issue(1)：46-49,4.DOI:10.13315/j.cnki.cjcep.2018.01.011
缺氧诱导因子-2α与MMP-2启动子结合位点的确定
Determination of the binding site of hypoxia inducible factor-2a to the MMP-2 promoter
摘要
Abstract
Purpose To identify the binding of hypoxia in-ducible factor-2a (HIF-2 a) to the hypoxia-response element(HRE) of the matrixmetallo proteinases-2 (MMP-2) gene promoter region and clear the binding site. Methods Electro-phoretic mobility shift assay (EMSA) was used to identify the binding of HIF-2 a to HRE of the MMP-2 gene promoter region in vitro. At the same time, the chromatin immunoprecipitation assay (CHIP) was used to further determine the binding site. Results Successful prediction of two potential HIF-2a binding sites of MMP-2 the promoter region, which were-217～-204 and-1 029 ～-1 007, respectively. Probe test shows that the marked efficiency of sense chain and antisense chain was above 50％, and they could be used for EMSA-electrophoretic mobility shift assay. The results of EMSA showed that there was a binding site of HIF-2 a sense chain and antisense chain moter region int-217～-204. The results of chromatin immuno-precipitation showed that in the experimental group and control group an about 250 bp fragment in MMP-2 promoter containing HRE region was amplified, suggesting that the protein of HIF-2a binded to the HRE in MMP-2 promoter region in vivo. Conclusion HIF-2 a in MMP-2 promoter regionne promoter region in vitro and in vivo.关键词
缺氧诱导因子2α/MMP-2/缺氧反应元件/MCF-7细胞Key words
hypoxia-inducible factor 2α/matrix metalloproteinase-2/hypoxia responsive element/MCF-7 cell分类
医药卫生引用本文复制引用
梁文辉,原志庆,王志慧,李娜..缺氧诱导因子-2α与MMP-2启动子结合位点的确定[J].临床与实验病理学杂志,2018,34(1):46-49,4.基金项目
河南省自然科学基金(162300410220)、河南省教育厅高等学校重点科研(16A310002、18A310004)、河南省卫生厅科技攻关资助(201403133) （162300410220）
