临床与实验病理学杂志2018，Vol.34Issue(2)：124-131,8.DOI:10.13315/j.cnki.cjcep.2018.02.002
结直肠癌中LC3与CD4+、CD8+、CD68+免疫细胞浸润的相关性及其临床意义
Correlation of LC3 and CD4 +,CD8 + and CD68+ cell infiltration in colorectal cancer and its clinical significance
摘要
Abstract
Purpose To observe the expressions of LC3, NY-ES0-2, MAGE-D4, CD4+, CD8+, CD68+ in colorectal cancer and normal tissues and analysis the correlation of autophagy related gene LC3 and tumor surface antigen NY-ES0-2, MAGE-D4, immune cells CD4+, CD8+, CD68 +. To investigate the effect of the change of autophagy on immune cells function and its clinical significance. Methods Immunohistochemistry and Western blot were used to detect the expressions of LC3, NY-ESO-2, MAGE-D4, CD4, CD8, CD68 in 128 cases of colorectal cancer and normal tissues. The correlation among each factors and the patients' clinicopathological features were analyzed. Results (1 ) The expression of LC3 in colorectal cancer tissue was higher than in normal tissues. The expression of NY-ESO-2 was low while the expression of MAGE-D4 was high in colorectal cancer and both almost not express in normal tissues(P＜0.05). The infiltration of CD4+, CD8 +, CD68+ immune cells in colorectal cancer were higher than in normal tissues(P＜0.05). (2)The expression of LC3 protein in colorectal cancer was correlated positively with the expressions of tumor surface antigen NY-ESO-2, MAGE-D4 protein and the infiltration of CD8 +, CD68 + immune cells (P＜ 0.05 ), but had no correlation with the infiltration of CD4 + immune cells (P＞0.05). (3 ) The expression of NY-ESO-2 was correlated positively with the infiltration of CD4+, CD8 +, CD68 + immune cells. The expression of MAGE-D4 was correlated positively with the infiltration of CD8 +, CD68+ immune cells. (4) The expressions of NY-ESO-2, MAGE-D4, the infiltration of CD4+, CD8 + immune cells and lymph node metastasis were negatively correlated (P＜0.05). The expressions of NY-ESO-2, MAGE-D4, the infiltration of CD4+, CD8+ immune cells and TNM stage were negatively correlated (P＜ 0.05). The infiltration of CD8 + immune cells and grade was positively correlated (P＜0.05). Conclusion The expression of autophagy-related gene LC3 was related to the expressions of tumor surface antigen NY-ESO-2, MAGE-D4 and the infiltration of immune cells CD8 + and CD68+ in colorectal cancer. Therefore the autophagy key factor LC3 may participate in the immune of colorectal cancer.关键词
结直肠肿瘤/自噬/肿瘤表面抗原/免疫细胞Key words
colorectal neoplasms/autophagy/tumor surfaceantigen/immune cell分类
医药卫生引用本文复制引用
刘梦瑶,吴淑华,温菲菲,张香芝,许晓阳,刘远航..结直肠癌中LC3与CD4+、CD8+、CD68+免疫细胞浸润的相关性及其临床意义[J].临床与实验病理学杂志,2018,34(2):124-131,8.基金项目
山东省科技发展计划项目(2010GSF10259) （2010GSF10259）
