临床与实验病理学杂志2018，Vol.34Issue(6)：591-594,600,5.DOI:10.13315/j.cnki.cjcep.2018.06.001
食管鳞状细胞癌中FOXM1基因的表达及临床意义
Expression of FOXM1 in patients with esophageal squamous carcinoma and its clinical significance
摘要
Abstract
Purpose Analysis of correlation between FOXM1 gene expression levels and clinicopathological features and prognosis of patients with esophageal squamous cancer (ESC). The effect of down-regulation of FOXM1 expression on the proliferation of human ESC cell line KYSE-30 was also inves-tigated. Methods Quantitative real-time PCR (qRT-PCR) and immunohistochemistry ( IHC) methods were used to detect the expression of FOXM1 in ESC tissues and non-cancer tissues in mRNA and protein level. The expression of FOXM1 was down-regulated by RNA interference (RNAi) technique, and the pro-liferation activity of KYSE-30 cells was detected by CCK-8 as-say. Results Compared with the corresponding non-cancer tis-sues, the expression of FOXM1 was significant higher in ESC tis-sues(P＜0. 01). Meanwhile, the expression levels of FOXM1 in poorly differentiated esophageal carcinoma was higher than that in well-differentiated ESC group ( P ＜0. 01 ). The expression of FOXM1 was significantly correlated with poor tumor differentia-tion (P＜0. 001), lymphatic metastasis (P=0. 000), advanced stage (P=0. 004) of ESC patients after surgical resection. High FOXM1 expression was related to shorter overall survival ( OS) (P＜0. 001). After down-regulating FOXM1 expression in KYSE-30 cells, cell proliferation rate was inhibited (P＜0. 01). Conclusion FOXM1 expression is up-regulated in ESC and is closely related to the degree of differentiation, lymph node me-tastasis, clinical stage and prognosis of ESC. FOXM1 may be participated in regulating the proliferation of human esophageal carcinoma cell line KYSE-30.关键词
食管肿瘤/鳞状细胞癌/FOXM1/预后Key words
esophageal neoplasms/squamous carcinoma/FOXM1/prognosis分类
医药卫生引用本文复制引用
李佩琴,杨少芬,翁雅倩,周春辉,于莉娜..食管鳞状细胞癌中FOXM1基因的表达及临床意义[J].临床与实验病理学杂志,2018,34(6):591-594,600,5.基金项目
国家自然科学基金(81472319 )、广东省自然科学基金(2014A030313325) （81472319 ）
