临床与实验病理学杂志2018，Vol.34Issue(6)：595-600,6.DOI:10.13315/j.cnki.cjcep.2018.06.002
LI-cadherin在进展期胃癌中的表达及对人胃癌细胞株MKN28的作用及其机制
Expression of LI-cadherin in advanced gastric cancer and its effect and mechanism on human gastric cancer cell line MKN28
摘要
Abstract
Purpose To investigate the expression of LI-cadherin in advanced gastric cancer and its effect and mecha-nism on human gastric cancer cell line MKN28. Methods Im-munohistochemical staining was performed on advanced gastric cancer to examine the LI-cadherin protein expression. CCK8, colony formation assay, transwell assay, flow cytometry were used to detect the changes in the biological behavior of MKN28 cells after LI-cadherin gene interference. Dual-luciferase report-er assay and Western blot were used to detect the activity of the NF-κB after the interference of LI-cadherin gene. Results LI-cadherin in chronic gastritis was negative, in well- and moder-ately-differentiated advanced gastric cancer was significantly in-creased, in poorly-differentiated was decreased. Cell prolifera-tion, colony formation, and invation ability were inhibited in the group of knockdown expression of LI-cadherin ( P ＜ 0. 05 ). Flow cytometry results showed that knockdown expression of LI-cadherin resulted in a significant increase in the proportion of cells in G1 phase, while the proportion of cells in S phase was significantly lower ( P ＜0. 05 ). Knockdown expression of LI-cadherin resulted in the decreased activation of NF-κB and re-stricted its translation. Conclusion LI-cadherin in well- and moderately-differentiated advanced gastric cancer is significantly increased. Knockdown of LI-cadherin can strongly decrease the malignant biological characteristics of human gastric cancer cell line MKN28. Its mechanism may be associated with the NF-κB pathway.关键词
胃肿瘤/进展期胃癌/LI-cadherin/机制Key words
stomach neoplasms/advanced gastric cancer/LI-cadherin/mechanism分类
医药卫生引用本文复制引用
林晶,江小杰,蔡清河,陈智伟..LI-cadherin在进展期胃癌中的表达及对人胃癌细胞株MKN28的作用及其机制[J].临床与实验病理学杂志,2018,34(6):595-600,6.基金项目
福建省中青年教师教育科研项目( A类) ( JA13289)、福建省卫生厅青年科研课题(2012-2-145)、福建省自然科学基金(2017J0105) （ A类）
