临床与实验病理学杂志2018，Vol.34Issue(6)：601-605,5.DOI:10.13315/j.cnki.cjcep.2018.06.003
胰腺癌中FFAR4蛋白和mRNA的表达及临床意义
Expression of FFAR4 protein and mRNA in pancreatic cancer and its clinical significance
摘要
Abstract
Purpose To detect the expression of FFAR4 protein and mRNA in pancreatic cancer and to discuss its role and significance in the progression of pancreatic cancer. Meth-ods Immunohistochemistry was used to detect the expression of FFAR4 protein in paraffin-embedded tissues from 62 cases of pathologically confirmed pancreatic cancer. The relationship be-tween the expression of FFAR4 and clinicopathological factors of pancreatic cancer was also studied. At the same time, the ex- pression of FFAR4 in 20 pairs of pancreatic cancer tissues and adjacent tissues were detected using Western bolt and real-time quantitative PCR (qRT-PCR). Results The results of immu-nohistochemistry showed that the expression rate of FFAR4 pro-tein in pancreatic carcinoma was 75. 8% (47/62) significantly higher than that in paratumor tissue 40. 3% (25/62), and the difference was statistically significant (P＜0. 05). The high ex-pression of FFAR4 was related to the degree of pancreatic cancer differentiation, TNM stage, and lymph node metastasis ( P ＜0. 05). Western blot and qRT-PCR showed that the expression of FFAR4 protein and its mRNA expression in pancreatic cancer tissues was significantly higher than matched paracancerous tis-sues. There was a statistically significant difference between the two groups ( P ＜0. 001 ). Conclusion The dysregulated ex-pression of FFAR4 may be closely related to the progression of pancreatic cancer. It is hopeful that FFAR4 may become a new marker for the prognosis of pancreatic cancer after surgery and a new target for the study of clinical therapeutic drugs.关键词
胰腺肿瘤/FFAR4/免疫组织化学/Western blot/qRT-PCRKey words
pancreatic neoplasms/FFAR4/immunohistochem-istry/Western blot/real-time fluorescent quantitative PCR分类
医药卫生引用本文复制引用
孟府陶,黄强,黄玫,邵峰,汪超,周杭城..胰腺癌中FFAR4蛋白和mRNA的表达及临床意义[J].临床与实验病理学杂志,2018,34(6):601-605,5.基金项目
国家自然科学基金(81501354) （81501354）
