中国临床保健杂志2018，Vol.21Issue(3)：383-388,6.DOI:10.3969/J.issn.1672－6790.2018.03.025
程序性细胞死亡蛋白4 在大鼠脑出血后血肿周围域的表达及作用
Expression and role of PDCD4 in the area adjacent to hematoma after intracerebral hemorrhage
摘要
Abstract
Objective To explore the expression,role and potential mechanism of PDCD4 in area adjacent to hematoma after intracerebral hemorrhage (ICH),and provide a molecular target for the treatment and neuronal recovery of ICH in future.Methods Protein levels were examined by western blot and immunohistochemistry ;the spatial location for proteins was detected by immunofluorescence ;siRNA transfection was used to intervene in the protein in the cells. Results PDCD4 expression was up－regulated in the area adjacent to hematoma after ICH ,and the peak of the level for PDCD4 was reached in the second day after ICH ;the up－regulated d PDCD4 expression had a co－localization with NeuN, and it also had a co－localization with neuronal apoptotic protein ,activated caspase－3;in the ICH model in vitro,neuronal apoptotic model induced by hemin ,the expression of activated caspase－3 was decreased,and the expression of the surviv－al factors p－Akt was increased,by intervening in the expression of PDCD 4 with siRNA.Conclusion The up－regulation of PDCD4 expression in the area adjacent to hematoma after ICH is associated with neuronal apoptosis ,which might be relevant to p－Akt signaling pathway.关键词
脑出血/血肿/程序性细胞死亡蛋白4/疾病模型,动物Key words
Cerebral hemorrhage/Hematoma/Programmed cell death 4/Disease models,animals分类
医药卫生引用本文复制引用
沈加兵,季宇腾,周婷婷,曹茂盛,陈颖,曹茂红,柯开富..程序性细胞死亡蛋白4 在大鼠脑出血后血肿周围域的表达及作用[J].中国临床保健杂志,2018,21(3):383-388,6.基金项目
国家自然科学基金资助(81671135) （81671135）
江苏省学术学位研究生科研创新计划项目资助(KYZZ16＿0356) （KYZZ16＿0356）
南通大学研究生科技创新项目资助(YKC16058) （YKC16058）
