日用化学工业2018，Vol.48Issue(5)：288-292,5.DOI:10.13218/j.cnki.csdc.2018.05.009
基于皮肤模型与THP-1细胞共培养建立皮肤致敏体外检测方法的初步研究
A preliminary study on in vitro skin sensitization test based on skin model and THP-1 cell co-culture
摘要
Abstract
An in vitro skin sensitization test based on skin model and THP-1 cell co-culture was established, and used to evaluate the sensitizing potency of petrochemicals. Skin model was treated with different concentrations of samples for 15 min, then removing the samples and was incubated for another 6 h.Skin tissue activity was measured by MTT assay, while cell viability and cell surface markers (CD54, CD86) expression levels of THP-1 cells were detected by flow cytometry. The skin sensitization potential of test substances was determined by statistically analyzing the above parameters and reaching a dose-response relationship. The results of SLS, cinnamaldehyde and citral show that this co-culture method can correctly distinguish irritants and sensitizers. The results of 4 unknown lubricant additives show that HVI60, Cuvan826 and ATM PAN are predicted to be non-sensitizers, while Irgamet30 is predicted to be a sensitizer, which at higher concentration can decrease the skin tissue activity and THP-1 cell viability, and specifically increase the expression of surface markers.关键词
皮肤致敏/皮肤模型/THP-1细胞/共培养Key words
skin sensitization/skin model/THP-1 cells/co-culture分类
医药卫生引用本文复制引用
陈健,柯逸晖,谈伟君..基于皮肤模型与THP-1细胞共培养建立皮肤致敏体外检测方法的初步研究[J].日用化学工业,2018,48(5):288-292,5.基金项目
广东省科技计划项目(2015A030402005) （2015A030402005）
