日用化学工业2018，Vol.48Issue(5)：299-302,4.DOI:10.13218/j.cnki.csdc.2018.05.011
HPLC-FLD/DAD双检测器串联测定面膜中10种荧光增白剂
Determination of ten fluorescent whitening agents in masks by HPLC-FLD/DAD with series double-detector
刘莹 1王小兵 1李启艳 1于海英 1徐丽华1
作者信息
- 1. 山东省食品药品检验研究院,山东 济南 250101
- 折叠
摘要
Abstract
To establish a new HPLC-FLD/DAD with series double-detector method for the determination of ten fluorescent whitening agents in masks. The sample was separated by Welch Ultimate LP C18(4. 6 mm × 250 mm, 3. 5 μm) column with the gradient elution of methanol-0. 05 mol/L ammonium dihydrogen phosphate solution as mobile phase at the flow rate of 1. 0 mL/min. The column temperature was set at 35. 0 ℃.The excitation wavelength and emission wavelength of fluorescence detector (FLD) were 360 and 430 nm, and the wavelength of diode array detector (DAD) was 360 nm. Results indicate that ten fluorescent whitening agents have good linear relationship in their linear range, with correlation coefficient greater than 0. 999 5. At the three spiked levels, the average recoveries of ten fluorescent whitening agents are in the range of 87. 6％-114. 2％ with the relative standard deviations (RSD, n = 6) less than 5. 0％. The detection limits of these ten fluorescent whitening agents are 0. 026-0. 40 mg/kg for FLD and 0. 50-2. 7 mg/kg for DAD.关键词
面膜/荧光增白剂/高效液相色谱法Key words
masks/fluorescent whitening agents/high performance liquid chromatography分类
化学工程引用本文复制引用
刘莹,王小兵,李启艳,于海英,徐丽华..HPLC-FLD/DAD双检测器串联测定面膜中10种荧光增白剂[J].日用化学工业,2018,48(5):299-302,4.
