水产学报2018，Vol.42Issue(6)：828-837,10.DOI:10.11964/jfc.20170610877
β-葡聚糖对低氧胁迫下大黄鱼幼鱼的保护作用及其机理
β-glucan decreases intensity of hypoxia-induced oxidative stress in large yellow croaker ( Larimichthys crocea) and its corresponding mechanisms
摘要
Abstract
The aim of the present study was to evaluate the effects of β-glucan on acute hypoxia-induced oxidative stress by determining lipid peroxidation, activities and mRNA levels of antioxidant enzymes (Cu/Zn-SOD, CAT, GPx and GR), and gene expressions of the Keap1-Nrf 2 signaling molecules in the liver of large yellow croaker Larimichthys crocea. Fish were injected with β-glucan at a dose of 0 or 5 mg/kg body weight on 6, 4 and 2 days before being exposed to 1.5 and 7.0 DO mg/ L for 24 h. The results showed that normoxia with β-glucan had no ef-fect on lipid peroxidation compared with the normoxic control, and hypoxic stress with β-glucan remarkably re-duced lipid peroxidation during the whole experiment compared with hypoxic stress, indicating that β-glucan could ameliorate hypoxia-induced lipid peroxidation. Obtained results also showed a coordinated transcriptional regula-tion of antioxidant genes, suggesting that Nrf 2 was required for regulating these genes. Furthermore, a negative re-lationship between the mRNA levels of Nrf 2 and Keap1 indicated that Keap1 plays an important role in switching off the Nrf 2 response. In conculsion, β-glucan could alleviate acute hypoxia-induced oxidative stress in large yel-low croaker, emphasizing a central role of transcription factor Nrf 2 in the process.关键词
大黄鱼/β-葡聚糖/氧化应激/Keap1-Nrf 2信号通路Key words
Larimichthys crocea/β-glucan/oxidative stress/Keap1-Nrf 2 signaling pathway分类
农业科技引用本文复制引用
王永红,张建设,曾霖..β-葡聚糖对低氧胁迫下大黄鱼幼鱼的保护作用及其机理[J].水产学报,2018,42(6):828-837,10.基金项目
浙江省自然科学基金(LY18D060008) Natural Science Foundation of Zhejiang Province (LY18D060008) （LY18D060008）
