水产学报2018，Vol.42Issue(6)：838-845,8.DOI:10.11964/jfc.20170410789
锌对团头鲂幼鱼血清1H NMR代谢组的影响
1H NMR-based metabolomics studies on the effect of Zn in blunt snout bream ( Megalobrama amblycephala)
摘要
Abstract
A 1H NMR-based metabolomics approach was used to explore the impact of dietary Zn on the serum metabolic profile of Megalobrama amblycephala. Fish were fed diets containing graded levels of Zn (7.4, 32.1, and 332.4 mg/kg diet; supplied as ZnSO4·7H2O) for 12 weeks. We identified 52 metabolites in serum including 23 amino acids or intermediary metabolites, 16 organic acids, 4 sugars, 4 nucleic acids, 3 vitamins and 2 others. Mul-tivariate data analyses (PLS-DA and VIP) showed dietary Zn affected the metabolic profile impartially and these metabolites (such as proline, lactate, glucose, alanine, lysine, glutamine, creatine, trans-4-hydrox-L-proline, taur-ine, serine, τ-methylhistidine, arginine, valine, methionine and mannose) were identified as charactisteristic meta-bolites. These metabolites are mainly increased in the groups (7.4 and 332.4 mg/kg diet) compared with the group (32.1 mg/kg), suggesting that Zn deficiency and excess inhibited amino acid and carbohydrate metabolism. In con-clusion, proline, lactate and glucose were found to have potentials for being new biomarkers for Zn nutritional status of M. amblycephala.关键词
团头鲂/锌/代谢组/脯氨酸/乳酸Key words
Megalobrama amblycephala/zinc/metabolomics/proline/lactate分类
生物科学引用本文复制引用
蒋明,杨长庚,王卫民,文华,吴凡,刘伟,田娟,陆星,喻丽娟..锌对团头鲂幼鱼血清1H NMR代谢组的影响[J].水产学报,2018,42(6):838-845,8.基金项目
国家大宗淡水鱼类产业技术体系专项(CARS-45-8) （CARS-45-8）
中央级公益性科研院所基本科研业务费专项(2013JBFM22) China Agriculture Research System (CARS-45-8) （2013JBFM22）
Central Public-interest Scientific Institution Basal Research Fund (2013JBFM22) （2013JBFM22）
