水产学报2018，Vol.42Issue(6)：854-862,9.DOI:10.11964/jfc.20170810931
Cu2+对中间球海胆生长、免疫及性腺发育的影响
Effects of Cu2+ on growth, immunity and gonad development of Strongylocentrotus intermedius
摘要
Abstract
This study was conducted to investigate the effects of Cu2+ on the weight gain rate (WGR), gonadosa-matic index (GI), antioxidant enzyme activities and transcription of major yolk protein gene (MYP) in Strongylo-centrotus intermedius. All experimental sea urchins were randomly divided into six tanks (200 L), and each tank was stocked with 20 individuals. Then, the test group was added CuSO4·5H2O to a final concentration of 0.02 mg/L Cu2+ with natural sea water as the control group. The experiment lasted for 60 days. Results showed that WGR and GI were significantly lower in the test group than those in the control group at 60 days. The activities of CAT and GST in the coelomic fluid of sea urchins in the test group were significantly lower at day 60 than those at day 30, and the activity of CAT was lower in the test group than that in the control group. The MYP transcription was sig-nificantly lower in the gonads of the test group than that in the control group, but the expression of MYP was signi-ficantly higher in the digestive tract of the test group than that in the control group. The total protein content was significantly higher in the coelomocytes of the test group than that in the control group. Results above showed that the WGR, GI and antioxidant enzyme activities of sea urchins could be reduced by Cu2+. This may be caused by in-hibiting MYP expression in the gonad and the MYP transportation from the digestive tract to the gonad.关键词
中间球海胆/增重率/性腺指数/抗氧化酶/主要卵黄蛋白Key words
Strongylocentrotus intermedius/weight gain rate/gonadosomatic index/antioxidant enzyme/major yolk protein分类
生物科学引用本文复制引用
李敏,吴反修,吕德亮,左然涛,常亚青..Cu2+对中间球海胆生长、免疫及性腺发育的影响[J].水产学报,2018,42(6):854-862,9.基金项目
国家自然科学基金(41606180) （41606180）
中国科协"青年人才托举工程"(YESS20150157) National Natural Science Foundation of China (41606180) （YESS20150157）
Young Elite Scientists Sponsor-ship Program By CAST (YESS20150157) （YESS20150157）
