水产学报2018，Vol.42Issue(6)：968-974,7.DOI:10.11964/jfc.20170710903
带鱼酶解蛋白亚铁螯合物对泥鳅免疫特性的影响
Effect of ferrous chelate Trichiurus lepturus protein on the immune characteristics of Misgurnus anguillicaudatus
摘要
Abstract
The test of the digestive enzyme activity, observation of intestinal tissue and the living imaging techno-logy were applied to investigate the effect of the ferrous chelating peptide on the digestive tract. The test of in-dexes of biochemical indexes of serum and liver, and observation of the liver were used to investigate the effect of the ferrous chelating peptide on immunological characteristics of Misgurnus anguillicaudatus. The result showed lipase activity was significantly higher than that of the control group when the ferrous chelating peptide was 2 g/kg; amylase activity of pepsin was significantly higher than the normal control group when the ferrous chelating peptide was 1 g/kg; trypsin activity in each experimental group compared with the control group had no signific-ant difference. SOD and lysozyme in serum were significantly higher than those of normal control group when the ferrous chelating peptide was 2 g/kg; compared with the normal control group, CAT at ferrous chelating peptide concentration of 1 g/kg showed significant difference. Compared with control group, the morphological structure of each experimental group had no change. With the application of living imaging technology, complexes of fer-rous chelating peptide with FITC marked stayed in M. anguillicaudatus for a long time and fluorescence intensity was mainly enriched in the upper body of M. anguillicaudatus, hence, after absorption, the ferrous chelating pep-tide was speculated to be distributed in the liver, gallbladder and other organs. The results showed that the hydro-lysis of ferrous chelate peptide could improve the digestive enzyme activities of loach and enhance non-specific immunity of loach.关键词
带鱼/泥鳅/亚铁螯合肽/消化道酶/免疫Key words
Trichiurus lepturus/Misgurnus anguillicaudatus/iron chelate peptide/digestive tract enzyme/im-munity分类
农业科技引用本文复制引用
林慧敏,袁宁,李颖杰,邓尚贵,张宾,霍健聪..带鱼酶解蛋白亚铁螯合物对泥鳅免疫特性的影响[J].水产学报,2018,42(6):968-974,7.基金项目
国家自然科学基金(31471609) （31471609）
浙江省自然科学基金(LY18C200007,LY18C200008,LY17C200011) National Natural Science Foundation of China (31471609) （LY18C200007,LY18C200008,LY17C200011）
Zhejiang Provincial Natural Sci-ence Foundation (LY18C200007, LY18C200008, LY17C200011) （LY18C200007, LY18C200008, LY17C200011）
