水产学报2018，Vol.42Issue(7)：1019-1025,7.DOI:10.11964/jfc.20170310769
龙须菜苯丙烷类抑藻活性物质的分离纯化及其对6种赤潮微藻的抑藻活性
Isolation and purification of phenylpropanoid antialgal substances from Gracilaria lemaneiformis and their growth inhibition effects on six species of red tide microalgae
摘要
Abstract
Four phenylpropanoid antialgal compounds (1-4) were successfully isolated from Gracilaria lemanei-formis through a combination of solvent soak, liquid-liquid extraction, silica gel column chromatography and re-peated preparative thin-layer chromatography. On the basis of the spectral data, the compounds were identified as benzene-1,2-propanoic acid (1), gossonorol (2), 7,10-epoxy-ar-bisabol-11-ol (3), and p-hydroxyphenylethanol (4). These 4 compounds were isolated from marine macroalgae for the first time. Further, a quantitative relationship between the inhibition of algal growth and the concentration of each phenylpropanoid antialgal compound was de-termined and important parameters for future practical HAB control, e.g. EC50-96 h, were also obtained. The results indicated that isolated compounds 1-4 possessed selective antialgal activity against the growth of several red tide microalgae (Amphidinium carterae , Heterosigma akashiwo, Karenia mikimitoi, Phaeocystis globosa, Proro-centrum donghaiense, and Skeletonema costatum). Their antialgal activity against the tested red tide microalgae has not been previously reported. Among them, benzene-1,2-propanoic acid (1), gossonorol (2) and p-hy-droxyphenylethanol (4) showed more extensive antialgal activities against the tested red tide microalgae. Further-more, benzene-1,2-propanoic acid (1) against A. carterae, H. akashiwo and P. globosa; gossonorol (2) and p-hy-droxyphenylethanol (4) against H. akashiwo and P. globosa; and 7,10-epoxy-ar-bisabol-11-ol (3) against H. akashiwo and K. mikimitoi possessed better application potential than potassium dichromate as a characteristic an-tialgal agent against these red tide microalgae.关键词
龙须菜/赤潮微藻/苯丙烷类抑藻活性物质/生长抑制作用/分离纯化Key words
Gracilaria lemaneiformis/red tide microalgae/phenylpropanoid antialgal substances/growth inhibi-tion/isolation and purification分类
资源环境引用本文复制引用
孙颖颖,周文静,郭赣林,浦寅芳,苏振霞..龙须菜苯丙烷类抑藻活性物质的分离纯化及其对6种赤潮微藻的抑藻活性[J].水产学报,2018,42(7):1019-1025,7.基金项目
国家自然科学基金(41606120) （41606120）
江苏省自然科学基金(BK2014044) （BK2014044）
