水产学报2018，Vol.42Issue(7)：1077-1084,8.DOI:10.11964/jfc.20171010994
坛紫菜自由丝状体响应琼胶寡糖的激发
Response of free-living conchocelis of Pyropia haitanensis to agaro-oligosaccharides
摘要
Abstract
This study was designed to research the response of free-living conchocelis growth and sporangium branches formation stage of free-living conchocelis of Pyropia haitanensis to agaro-oligosaccharide. After being treated with agaro-oligosaccharide, the net photosynthetic rate of free-living conchocelis of P. haitanesis was measured by liquid phase electrode; the concentration of H2O2in the medium was determined by the dimerization of (p-hydroxyphenylethyl) acetic acid; the floridoside content was detected by LC-MS; the H2O2related genes (Phrboh, PhSOD) and the floridoside synthesis related genes (Phnho1, Phgpdh, Phtps) expression was detected by real-time PCR; the number of sporangium branches was examined by microscopy. The results showed that the agaro-oligosaccharides can stimulate a series of responses of free-living conchocelis of P. haitanesis. These re-sponses include increased photosynthetic rate; increase the content and biosynthesis of photosynthetic products of floridoside; the release of H2O2continues to increase which was associated with the activation of two enzymes as-sociated with H2O2gene Phrboh and PhSOD. In addition, agaro-oligosaccharides can also promote the develop-ment of free-living conchocelis of P. haitanesis. On thirtieth day of culture, the sporangium branches formation rate of agaro-oligosaccharides treatment group reached 59%, significantly higher than the control group 46%. In conclusion, agaro-oligosaccharide can increase the free-living conchocelis of P. haitanesis photosynthetic rate and photosynthetic products, and can increase the expression of H2O2synthesis enzyme to induce the release of ROS. Meanwhile, agaro-oligosaccharides can promote the reproduction of free-living conchocelis of P. haitanesis.关键词
坛紫菜/琼胶寡糖/自由丝状体/响应Key words
Porphyra haitanensis/agaro-oligosaccharides/free-living conchocelis/response分类
农业科技引用本文复制引用
杜莉,杨锐,骆其君,严小军,陈海敏..坛紫菜自由丝状体响应琼胶寡糖的激发[J].水产学报,2018,42(7):1077-1084,8.基金项目
现代农业产业技术体系专项(CARS-50) （CARS-50）
浙江省重大科技专项(2016C02055-6B) （2016C02055-6B）
宁波市科技计划项目(2017C110026,2017C10020) （2017C110026,2017C10020）
