山东农业科学2018，Vol.50Issue(4)：12-15,4.DOI:10.14083/j.issn.1001-4942.2018.04.003
生防解淀粉芽孢杆菌Bs-18 TnYLB-1突变体文库的构建
Construction of Mutant Library of Bacillus amyloliquefaciens Bs-18 with the Transposon TnYLB-1
摘要
Abstract
Bacillus amyloliquefaciens Bs-18 is a biocontrol strains against cucumber powdery mildew with significant control effect. In this paper, the mutant library of Bs-18 was constructed with the shuttle plasmid pMarA carrying transposon TnYLB-1 by the electric shock method to deeply study the molecular mechanism of the biocontrol strain. Through screening transpositional temperature and time, the maximum transpositional success rate was obtained after induced at 50℃ for 16 hours. The mutants were verified by PCR. The construction of Bs-18 mutant library laid a foundation for further study on its biocontrol function genes and mechanism.关键词
突变体文库/转座子/生防功能基因Key words
Mutant library/Transposon/Biocontrol function genes分类
农业科技引用本文复制引用
孙淑琴,孙冰冰,杨秀荣,张惟,霍建飞..生防解淀粉芽孢杆菌Bs-18 TnYLB-1突变体文库的构建[J].山东农业科学,2018,50(4):12-15,4.基金项目
天津市自然科学基金项目"解淀粉芽孢杆菌Bs-18生防相关基因的鉴定及功能分析"(16JCYBJC29500) （16JCYBJC29500）
