山东农业科学2018，Vol.50Issue(6)：1-9,9.DOI:10.14083/j.issn.1001-4942.2018.06.001
花生FAD基因家族的全基因组鉴定与表达模式分析
Genome-Wide Identification and Expression Pattern Analysis of Peanut FAD Gene Family
摘要
Abstract
Fatty acid desaturase (FAD)is a key enzyme in the synthesis of unsaturated fatty acids, which plays an important role in maintenance of various life activities of plants and in response to biotic and abiotic stresses.In this study,the peanut FAD (AhFAD)gene family were identified and conducted bioinfor-matics analysis in genome-wide range,and the expression pattern and alternative splicing forms of AhFADs were analyzed using peanut transcriptome data.The results showed that there were 3 1 AhFAD genes in peanut genome,which could be divided into 6 subfamilies.There were 12 members in AhSAD subfamily,6 members in AhFAD2 subfamily,4 members in AhFAD3 subfamily,3 members in AhFAD4/5 subfamily,2 members in AhFAD6 subfamily and 4 members in AhFAD7/8 subfamily.Clustering analysis showed that there were two major branches of AhFAD:free and membrane -binding AhFAD branch.In membrane -binding AhFAD branch,the origin of AhFAD4/5 was the earliest,the ω-3 AhFAD was evolved from ω-6 AhFAD,and the mono-dicotyledonous AhFAD was clustered in different branches.The expression levels of AhSAD,AhFAD2 and AhFAD3 in seeds were higher,while AhFAD4/5,AhFAD6 and AhFAD7/8 had the highest expression in leaves.The analysis of alternative splicing showed that there were significant differences in alternative splicing forms among the subfamilies of AhFAD,of which,12 AhFAD genes had alternative splicing isoforms.TTS and TSS were the most frequent alternative splicing events,followed by IR,and the fewest ones were AE and ES.关键词
花生/脂肪酸去饱和酶(FAD)/生物信息学分析/表达模式/可变剪接Key words
Peanut/Fatty acid desaturase (FAD)/Bioinformatics analysis/Expression pattern/Alter-native splicing分类
农业科技引用本文复制引用
阮建,单雷,李新国,郭峰,孟静静,万书波,彭振英..花生FAD基因家族的全基因组鉴定与表达模式分析[J].山东农业科学,2018,50(6):1-9,9.基金项目
国家自然科学基金项目(31470349,31571605) （31470349,31571605）
国家科技支撑计划项目(2014BAD11B04) （2014BAD11B04）
国家国际科技合作专项(2015DFA31190) （2015DFA31190）
国家现代农业产业技术体系建设专项(CARS-13) （CARS-13）
