山东农业科学2018，Vol.50Issue(6)：35-40,6.DOI:10.14083/j.issn.1001-4942.2018.06.005
栽培种花生FAE1基因的克隆和序列分析
Cloning and Sequence Analysis of FAE1 Gene in Cultivated Peanut
摘要
Abstract
Fatty acid elongation 1 (FAE1)is a key enzyme that controls the elongation of fatty acids and plays a crucial role in the biosynthesis of erucic acid.In this study,two FAE1 genes,FAE1 -A and FAE1 -B,were cloned from two genomes of the cultivated peanut,respectively.FAE1 had only one open reading frame,no introns,and the complete coding sequence of the gene is 1 536 bp,encoding 511 amino acids.The bioinformatics analysis results showed that the secondary structure of FAE1 protein was mainly α-helix;there was no signal peptide,indicating that FAE1 was not secretory protein,and it worked in cells and mainly exis-ted in endoplasmic reticulum;the instability coefficient of FAE1 was lower than 40 and the total mean hydro-philic coefficient was negative,so FAE1 was unstable hydrophilic protein.The study may facilitate peanut breeding for low erucic acid content.关键词
FAE1/芥酸/花生/基因克隆/生物信息学分析Key words
FAE1/Erucic acid/Peanut/Gene cloning/Bioinformatics analysis分类
农业科技引用本文复制引用
张欣,殷冬梅,王传堂,孙全喜,吴琪,王秀贞,胡东青,唐月异,王志伟,宋国生,徐建志..栽培种花生FAE1基因的克隆和序列分析[J].山东农业科学,2018,50(6):35-40,6.基金项目
国家花生产业技术体系项目(CARS-13) （CARS-13）
山东省农业科学院重大科技成果培育计划项目(2014CGPY09) （2014CGPY09）
