山东农业科学2018，Vol.50Issue(6)：72-78,7.DOI:10.14083/j.issn.1001-4942.2018.06.012
转AhDREB1基因花生T2代植株耐盐性分析
Salt-Tolerance Analysis of T2Transgenic Peanut Plants of AhDREB1
摘要
Abstract
As a specific transcription factor in plants,DREB1 (dehydration responsive element binding protein)plays an important role in regulating the expression of stress-induced related genes and involves in the response and adaptation of plants to salt stress.In this study,T2transgenic peanut plants of AhDREB1 and its receptor Fenghua 2 (control)were used as the materials.The seedlings were conducted salt stress treat-ment with 1% NaCl,and the changes of phenotype and gene expression before and after stress treatment were analyzed.The physiological indexes such as activities of SOD and POD,contents of soluble protein and MDA were measured.The results showed that under salt stress,the green degree and root growth of transgenic plants were better than those of control.The expression level of DREB1 in transgenic plants increased and the re-sponse to salt stress was faster than that of control.The activities of SOD and POD and the content of soluble protein of transgenic plants were higher than those of control,and the content of MDA was lower.These results indicated that overexpression of AhDREB1 could enhance the salt tolerance of transgenic peanut plants.Four transgenic plants with better salt tolerance were screened,namely D254,D380,D385 and D448.关键词
花生/DREB1/表型/表达量/生理指标/耐盐性Key words
Peanut/DREB1 /Phenotype/Expression quantity/Physiological index/Salt tolerance分类
农业科技引用本文复制引用
郭瑞杰,李文,刘风珍,张昆,万勇善..转AhDREB1基因花生T2代植株耐盐性分析[J].山东农业科学,2018,50(6):72-78,7.基金项目
国家现代农业产业技术体系建设专项(CARS-13) （CARS-13）
山东省现代农业产业技术体系花生创新团队建设项目(SDAIT-04-03) （SDAIT-04-03）
山东省花生良种产业化工程项目 （）
山东省农业科学院农业科技创新工程项目(CXGC2016B02) （CXGC2016B02）
