山东医药2018，Vol.58Issue(10)：9-12,4.DOI:10.3969/j.issn.1002-266X.2018.10.003
沉默ADAM17基因对人甲状腺乳头状癌细胞增殖、迁移及侵袭的影响
Effects of silencing ADAM17 on proliferation,migration,and invasion of human thyroid papillary carcinoma cells
摘要
Abstract
Objective To study the effects of silencing ADAM17 on the proliferation,migration,and invasion of human thyroid papillary carcinoma K1 cells. Methods K1 cells were divided into the control group,nonsense shRNA group,and ADAM17-shRNA group. Nonsense-shRNA and ADAM17-shRNA were transfected into K1 cells of the nonsense shRNA group and ADAM17-shRNA group by lentiviral transfection technology. The same amount of PBS was added into the control group. The expression levels of ADAM17 were detected by RT-PCR and Western blotting. MTT assay and flow cytometry were used to assess the cell proliferation ability and the cell cycle. The cell migration and invasion abilities were detected by Transwell migration and invasion assay,respectively. Results Compared with the control group and nonsenseshRNA group,the mRNA and protein expression levels of ADAM17 significantly decreased in the ADAM17-shRNA group(all P < 0.05); the optical density (OD value) of the ADAM17-shRNA group at 24,48,and 72 h was significantly lower that that of the control group and nonsense-shRNA group (all P < 0.05); in the ADAM17-shRNA group,the cells arrested in G0/G1 phase,and significant difference was found between the ADAM17-shRNA group and the control group and nonsense-shRNA group (all P < 0.05); compared with the control group and nonsense-shRNA group,the transmembrane cells decreased sharply in the ADAM17-shRNA group (all P < 0.05). Conclusion Silencing ADAM17 can inhibit the proliferation, migration,and invasion of K1 cells.关键词
甲状腺乳头状癌/解聚素-金属蛋白酶17/细胞增殖/细胞迁移/细胞侵袭Key words
papillary thyroid carcinoma/a disintegrin and metalloprotease 17/cell proliferation/cell migration/cell invasion分类
医药卫生引用本文复制引用
葛志鹏,张博,王晓涛,黄志强,张琪,田炜,张国志..沉默ADAM17基因对人甲状腺乳头状癌细胞增殖、迁移及侵袭的影响[J].山东医药,2018,58(10):9-12,4.基金项目
河北省卫计委科技成果推广课题(20150523). （20150523）
