山东医药2018，Vol.58Issue(10)：13-16,4.DOI:10.3969/j.issn.1002-266X.2018.10.004
Sphk1在舌鳞状细胞癌组织中的表达及其对舌癌细胞生物学行为的影响
Expression of SphK1 in tongue squamous cell carcinoma and its effect on tumor cell biological behavior
摘要
Abstract
Objective To study the expression and significance of sphingosine kinase 1 (SphK1) in the tongue squamous cell carcinoma (TSCC) tissues and to observe the effects of SphK1 on the proliferation,invasion,and migration of tongue cancer Tca8113P160 cells. Methods Immunohistochemistry was used to detect the expression of Sphk1 in TSCC(50 cases),leukoplakia (28 cases),and normal tongue mucosa (10 cases) specimens. The correlation between Sphk1 expression and clinical features was analyzed. Tca8113P160 cells were divided into two groups: the N,N-dimethylsphingosine(DMS) group which was treated with Sphk1 inhibitor DMS,and the control group which was added with medium with no medication. The proliferation of cells was detected by CCK-8 at 24,48,and 72 h. The migration and invasion of cells were detected by Transwell assay. The protein expression of SphK1,N-cadherin,and E-cadherin was detected by Western blotting. Results The high expression rate of Sphk1 in TSCC tissues was higher than that in leukoplakia and normal mucosa tissues (all P < 0.01),which was correlated with clinical stage,lymph node metastasis,T stage,and histological differentiation (all P < 0.05). After incubation for 24,48,and 72 h,the OD value of DMS group was lower than that in the control group (all P < 0.05). The migration and invasion assay showed the number of transmembrane cells in the DMS group was significantly less than that in the control group (P < 0.01). The expression of Sphk1 and N-cadherin of the DMS group was lower,while the expression of E-cadherin protein was higher than that in the control group (all P <0.05). Conclusions Sphk1 is highly expressed in TSCC,and the down-regulation of Sphk1 can inhibit the proliferation, invasion,and migration of Tca8113P160 cells and reverse the epithelial-mesenchymal transition. Sphk1 may be a potential target for TSCC treatment.关键词
舌鳞状细胞癌/鞘氨醇激酶1/细胞增殖/细胞迁移/细胞侵袭/上皮间质转化Key words
tongue squamous cell carcinoma/sphingosine kinase 1/cell proliferation/cell migration/cell invasion/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
张惠敏,吴德报,向旭,杨立,沈军..Sphk1在舌鳞状细胞癌组织中的表达及其对舌癌细胞生物学行为的影响[J].山东医药,2018,58(10):13-16,4.基金项目
天津市卫生公关课题(06KG04) （06KG04）
天津市卫生局科技基金(2012KZ037). （2012KZ037）
