摘要
Abstract
Objective To observe the effects of interleukin-17A (IL-17A) on the secretion of keratin 17 (K17) from human immortalized keratinocyte cell line (HaCaT) cultured in vitro and the signal transducer and activator of transcription 3 (STAT3) signaling pathway,and to provide a theoretical basis for exploring the pathogenesis and targeted treatment of psoriasis. Methods HaCaT cells cultured in RPMI1640 culture medium were randomly divided into three groups: blank control group,induction group,and inhibitor group. The cells in the blank control group were treated with DMEM high-glucose medium only,cells in the induction group were treated with DMEM high-glucose medium containing 50 μg/L IL-17A, and cells in the inhibitor group were treated with a inhibitor (Piceatannol) containing 10 μmol/L STAT3 + the DMEM highglucose medium containing 50 μg/L IL-17A. The cultured HaCaT cells were collected,MTT assay was used to detect the proliferation of cells,flow cytometry (FCM) was used to detect the apoptosis rate,the expression of K17 mRNA was detected by RT-PCR,and Western blotting was used to detect the protein expression of K17 and phosphorylated STAT3 (p-STAT3). Results The A value of cell proliferation in the induction group was higher than that in the blank control group and the induction group,and the apoptosis rate was lower than that in the blank control group and the inhibitor group (both P < 0.01); the expression of K17 mRNA and the protein level of K17 and p-STAT3 in the induction group were both higher than those of the blank control group and the inhibitor group (all P < 0.01). Conclusion IL-17A can up-regulate the expression of K17 in HaCaT cells cultured in vitro,and its regulation mechanism may be achieved through the activation of STAT3,indicating that the role of IL-17A in psoriasis may be related to K17.关键词
白细胞介素-17A/角质形成细胞/角蛋白17/信号转导和转录激活因子3/银屑病Key words
interleukin-17A/keratinocyte/keratin 17/signal transducer and activator of transcription 3/psoriasis分类
医药卫生
