陕西中医2018，Vol.39Issue(7)：819-822,4.DOI:10.3969/j.issn.1000-7369.2018.07.001
番石榴叶抑制db/db小鼠肾脏TGF-β1蛋白表达研究
Guava leaf extracts decrease renal tissue TGF-β1 Immunohistochemistry expression in db/db mice
摘要
Abstract
Objective :To observe treatment of Guava leaf extracts on renal tissue TGF-β1 protein expression in db/db mice .Methods :16 male obese db/db mice ,10 weeks old ,were randomly divided into control group and Guava leaf water extracts (0 .11g/kg) after being fed for 2 weeks .All mice received high fat diet and 12-week treat-ment .Body weight and glucose were tested every week .HE staining according to the manufacturers' instructions . Renal tissue TGF-β1 protein was tested by immunohistochemistry ,The relative levels of each target protein to con-trol β-actin were determined by densitometry analysis using the Image-Pro Plus .Results :GL extracts significantly lowered the values of body weight and plasma glucose ,reduced the proliferation of glomerular mesangial cells ,prolif-eration of peripheral organs ,and granular degeneration .Furthermore ,treatment with GL extracts decreased renal tis-sue TGF-β1 protein expression by immunohistochemistry .Conclusion :GL extracts may improve fibrosis by decrease TGF-β1 signaling pathway in renal tissue .关键词
糖尿病肾病/番石榴叶/肾脏/T/GF-β1/动物实验/小鼠Key words
Diabetic nephropathies/Guava leaf/Kidney/TGF-β1/Animal experimentation/Mice分类
医药卫生引用本文复制引用
段颖,郭翔宇,刘铜华,孙文,许光远,董笑克,丁雷..番石榴叶抑制db/db小鼠肾脏TGF-β1蛋白表达研究[J].陕西中医,2018,39(7):819-822,4.基金项目
国家自然科学基金资助项目(81303130)东方医院东方新星项目(DFRC2014C06) （81303130）
