同济大学学报（医学版）2018，Vol.39Issue(3)：19-23,5.DOI:10.16118/j.1008-0392.2018.03.004
烟曲霉对肺微血管内皮细胞通透性的影响及可能机制
Effect of Aspergillus fumigatus on pulmonary microvascular endothelial cell permeability and its mechanism
摘要
Abstract
Objective To investigate the effect of Aspergillus fumigatus ( AF ) on human pulmonary microvascular endothelial cell ( HPMVEC ) permeability and the related mechanisms. Methods HPMVECs were treated with AF, cell F-actin was observed with confocal laser scanning microscopy and cell transmembrane resistance was measured. In addition, the cell permeability changes in AF-treated HPMVECs were also examined with intervention of p38MAPK inhibitor SB203580 ( 20 μmol/L ) , ROCK inhibitor Y27632 ( 20 μmol/L ) , or PKC inhibitor LY317615 ( 10 μmol/L) , respectively. Results AF treatment induced a significant reduction of F-actin fluorescence intensity ( P＜0.01) and a significant reduction of cell transmembrane resistance ( P＜0.01 ) in HPMVECs. Furthermore, treatment with SB203580, Y27632 or LY317615 significantly inhibited ( P＜0.05) AF-induced increase of cell transmbrane resistance in HPMVECs.Conclusion AF can increase cell permeability in HMVECs, in which the MAPK (p38), ROCK and PKC pathways may be involved.关键词
烟曲霉/肺微血管内皮细胞/细胞通透性Key words
Aspergillus fumigatus/pulmonary microvascular endothelial cells/cell permeability分类
医药卫生引用本文复制引用
宋珺,韩菁,孙越,施伟民..烟曲霉对肺微血管内皮细胞通透性的影响及可能机制[J].同济大学学报（医学版）,2018,39(3):19-23,5.基金项目
国家重点基础研究发展计划(973计划)(2013CB531604) （973计划）
上海交通大学附属第一人民医院院级项目(11B06) （11B06）
