同济大学学报（医学版）2018，Vol.39Issue(3)：30-35,6.DOI:10.16118/j.1008-0392.2018.03.006
糖化终末产物对人结肠癌HCT116细胞中ChREBP表达的影响
Advanced glycation end productsup-regulate ChREBP expression in human colorectal cancer HCT116 cells
摘要
Abstract
Objective To study the effects of advanced glycation end products ( AGEs ) on expression of carbohydrate responsive element binding protein ( ChREBP) in human colorectal cancer HCT116 cells. Methods The expression of ChREBP mRNA in AGEs-treated human colorectal cancer HCT116 cells was detected with RT-PCR. After treatment with antioxidant N-Acetyl-L-cysteine( NAC ) , cell proliferation and ChREBP expression were examined with MTS assay and Western blot in AGEs-treated HCT116 cells, respectively. Results The expression of ChREBP mRNA was increased in AGE-treated HCT116 cells ( P＜0. 05 ). ChREBP expression and cell proliferation were inhibited in AGE-treated HCT116 cells after NAC treatment ( P ＜ 0. 05 ). Conclusion AGEs up-regulate ChREBP expression in human colorectal cancer HCT116 cells through activation of oxidative stress.关键词
糖化终末产物/人结肠癌HCT116细胞/碳水化合物反应元件结合蛋白Key words
advanced glycation end products/HCT116 colorectal cancer cells/carbohydrate responsive element binding protein分类
医药卫生引用本文复制引用
陈寒蓓,林宁,苏青..糖化终末产物对人结肠癌HCT116细胞中ChREBP表达的影响[J].同济大学学报（医学版）,2018,39(3):30-35,6.基金项目
国家自然科学基金青年项目(81400799) （81400799）
