同济大学学报（医学版）2018，Vol.39Issue(3)：36-40,5.DOI:10.16118/j.1008-0392.2018.03.007
内源性硫化氢抗氧化作用对乙酸溃疡性结肠炎模型小鼠黏膜损伤的影响
Effect of endogenous hydrogen sulfide on acetic acid-induced ulcerative colitis in mice
梁慧洁 1陈尼维 1赵祥运 1朱梅影 1伏桂香 1闫厚煜2
作者信息
- 1. 上海交通大学附属第六人民医院消化内科,上海 310000
- 2. 厦门市第一医院,福建 厦门 361011
- 折叠
摘要
Abstract
Objective To investigate the effect of endogenous hydrogen sulfide( H2S) on acetic acid-induced ulcerative colitis in mice. Methods Forty Kunming mice were randomly divided into control group, model group, sodium hydrosulfide ( NaHS) group and DL-propargylglycine ( PAG) group. The ulcerative colitis model was induced by a single enema of 8% acetic acid (0.15 mL) after 12 h of fasting. The H2S donor NaHS (50 μmol/kg), Cystathionine-γ-lyase (CSE) inhibitor PAG (10 mg/kg) and normal saline were intraperitoneally injected in mice of NaHS group, PAG group and model group 24 h after modelling, respectively; and the disease activity index ( DAI) score was evaluated. The animals were sacrificed after 3d, the macroscopic damage score and the pathological damage score of colon lesion tissues were assessed. The malondialdehyde ( MDA) ,superoxide dismutase ( SOD) and glutathione ( GSH) in colon lesion tissue and H2S contents in serum were measured with biochemical methods; and the hemeoxygenase-1 ( HO-1) ,NADH dehydrogenase quinone 1 ( NQO-1) in the colon lesions were measured by the ELISA kits. The expressions of the CSE in colon lesion was detected by Western blotting.Results Compared with the control group, the DAI scores, the macroscopic damage score, the pathological damage score, and the content of the MDA in the model group, NaHS group and PAG group were significantly increased ( P＜0. 05) , while those in NaHS group were lower than those in model group ( P＜0. 05) , and those in PAG group were significantly higher than those in NaHS and model groups ( P＜0. 05). The contents of the serum H2S and the expression of CSE in the colon tissues of PAG group were significantly lower than those in other three groups ( P＜0. 05). Compared with the control group and NaHS group, the serum H2S level in the model group were decreased ( P＜0. 05), while expression of the CSE in the colon tissues was increased (P＜0. 05). The contents of HO-1, NQO-1, GSH and SOD activity in the model group were significantly lower than those in control group ( P＜0. 05) , while those in NaHS group were significantly higher than those in control group ( P＜0. 05) , and those in PAG group were significantly lower than those in NaHS and control groups ( P＜0. 05). Conclusion The endogenous H2S can inhibit the intestinal mucosal injury in mice with acetic acid-induced ulcerative colitis, which may be related to its enhancing effect on antioxidant enzyme activity.关键词
溃疡性结肠炎/硫化氢/乙酸/抗氧化酶Key words
ulcerative colitis/hydrogen sulfide/acetic acid/antioxidase分类
医药卫生引用本文复制引用
梁慧洁,陈尼维,赵祥运,朱梅影,伏桂香,闫厚煜..内源性硫化氢抗氧化作用对乙酸溃疡性结肠炎模型小鼠黏膜损伤的影响[J].同济大学学报（医学版）,2018,39(3):36-40,5.
