世界中医药2018，Vol.13Issue(6)：1347-1353,7.DOI:10.3969/j.issn.1673-7202.2018.06.010
糖肾方对db/db小鼠肾脏纤维化的保护作用
Protective Effects of Tangshen Formula in Renal Fibrosis in db/db Mice
摘要
Abstract
Objective:To observe the effects of Tangshen Formula ( TSF) treatment on renal fibrosis and explore its potential mechanism in db/db mice.Methods:Eight weeks old db/db and db/m mice were randomly divided into three subgroups ,(db/m, db/db,db/db+TSF),and all mice were fed with a normal diet.After two weeks adaptation,mice were maintained on TSF treat－ment for 12 weeks and were then sacrificed under anesthesia after overnight fast .Animal urine and renal tissues samples were col－lected for further analyses.The kidneys were washed with cold PBS solution ; one part of the renal tissue were stored in liquid nitro－gen,and another deposited －80 ℃directly for subsequent frozen sections ; the rest of the kidney were fixed into the neutral forma－lin for pathological analysis.The renal fibrosis was detected by PAS and Masson staining.The renal expression of TGF－β1/Smad3 and its target genes were assessed by Western Blotting ,real－time PCR,and IHC.Results:TSF diminished UACR of db/db mice. Histological analysis using PAS and Masson staining revealed the occurrence of mesangial matrix expansion and extracellular matrix deposition in the kidneys of db/db mice.The treatment with TSF significantly ameliorated these histological renal injuries in db /db mice.Western Blotting and real－time PCR analysis showed that expression levels of TGF－β1,Smad3,Collagen Ⅰ and Fibronectin were significantly downregulated in the db/db mice with TSF treatment.With TSF treatment,the expression levels of miRNA 21 was downregulated,but the expression levels of miRNA 29b was upregulated.Conclusion:TSF attenuated renal fibrosis in db/db mice, through suppression of TGF－β1/Smad3 pathway.关键词
糖肾方/糖尿病肾病/肾脏纤维化/miRNAKey words
Tangshen formula/Diabetic nephropathy/Renal fibrosis/miRNA分类
医药卫生引用本文复制引用
刘鹏,陈姣伊,刘言振,赵婷婷,张浩军,严美花,赵海玲,李平..糖肾方对db/db小鼠肾脏纤维化的保护作用[J].世界中医药,2018,13(6):1347-1353,7.基金项目
国家自然科学基金国际(地区)合作与交流项目(81620108031) （地区）
科技部国际合作项目(2011DFA31860) （2011DFA31860）
北京市科委"十病十药"专项项目(Z151100003815015) （Z151100003815015）
