色谱2018，Vol.36Issue(7)：599-607,9.DOI:10.3724/SP.J.1123.2018.01003
基于微流控芯片的72重单核苷酸多态性族群推断系统的构建
Construction of a microfluidic chip-based 72 plex single nucleotide polymorphisms ancestry inference system
摘要
Abstract
The aim was to develop an autosomal single nucleotide polymorphism( SNP)multi-plex detection chip system for the inference of the ancestry of unknown individuals.On the basis of the 74-ancestry informative SNP( AISNP)panel screened by our group,a multiplex SNP amplification and detection system,based on a microfluidic chip of competitive allele-specific polymerase chain reaction( PCR),was constructed.The allele-specific primers of each SNP were positioned into a single well on the microfluidic chip and each chip included 112 wells.For detection of SNPs,the chip was placed on the plate thermocycler to perform PCR.The fluorescence signal of the reaction in each well was detected by a confocal laser scanning instrument to evaluate the SNP genotypes of each sample.Fifty-two samples were used for SNP typing.The accuracy of typing results was 100%.Taking 3 628 samples of 57 populations as the reference population database,the ancestry of 20 samples was inferred.The results were con-sistent with the actual sources of the samples.The autosomal 72-SNP microfluidic chip system established can accurately detect SNPs.The ancestry of tested individuals can be accurately inferred from the reference database.关键词
族群推断/单核苷酸多态性复合体系/微流控芯片Key words
ancestry inference/single nucleotide polymorphism complex system/microfluidic chip分类
化学化工引用本文复制引用
任苹,刘京,蔺日胜,刘杨,黄美莎,胡胜,徐友春,李彩霞..基于微流控芯片的72重单核苷酸多态性族群推断系统的构建[J].色谱,2018,36(7):599-607,9.基金项目
国家重点研发计划( 2017YFC0803501 ) （ 2017YFC0803501 ）
国家自然科学基金项目( 81772027 ) （ 81772027 ）
基本科研业务费专项资金项目(2016JB039) （2016JB039）
公安部物证鉴定中心协同创新工作项目(2017XTCX01) （2017XTCX01）
公安科技成果推广引导计划(2016TGYDGAES14).National Key Plan(No.2017YFC0803501) （2016TGYDGAES14）
National Natural Science Foundation of China(No.81772027) （No.81772027）
Fundamental Research Funds( No.2016JB039) （ No.2016JB039）
Collaborative Innovation Project for Institute of Forensic Science ( No.2017XTCX01 ) （ No.2017XTCX01 ）
Public Security Science and Technology Promotion Guide Plan ( No.2016TGYDGAES14). （ No.2016TGYDGAES14）
