色谱2018，Vol.36Issue(7)：621-628,8.DOI:10.3724/SP.J.1123.2018.01024
超高效液相色谱-串联质谱法同时测定牛肉中18种食源性兴奋剂类药物残留
Simultaneous determination of 18 food-borne stimulant drug residues in beef using ultra-high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method for the simultaneous determination of 18 food-borne stimulant drug resi-dues in beef was developed based on ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).The beef sample was extracted with acidified acetonitrile, cleaned up by Captiva filtration cartridge.The extract was dried with anhydrous magnesium sulfate,and then concentrated by nitrogen flow.The obtained residue was re-dissolved in methanol-water(7∶3,v/v).The separation was performed on an Agilent Zorbax Phenyl-Hexyl column with 5 mmol/L ammonium acetate solution( containing 0.01%( v/v)acetic acid)and methanol-acetonitrile( 7 ∶3,v/v ) as mobile phases with gradient elution.The analyte was detected in positive and negative ion modes and the multiple reaction monitoring( MRM)mode.The quantification analysis was performed by external standard method using matrix-matched calibration curves.The method was linear with the correlation coefficients(R2)≥0.9950 in the range of 0.10-50 μg/L.At the spiked levels of 0.4,1.0 and 2.0 μg/kg,the recoveries of all compounds ranged from 57.3% to 117.5%,with RSDs in range of 3.1%-15.6%( n=5).The limits of detection and limits of quantification were in the range of 0.000 6-0.090 0 μg/kg and 0.002 0-0.300 0 μg/kg,respectively.The method is simple,rapid,accurate and sensitive,and can meet the requirement for the determination of the 18 food-borne stimulant drug residues in beef.关键词
超高效液相色谱-串联质谱/食源性兴奋剂类药物/残留/牛肉组织Key words
ultra-high performance liquid chromatography-tandem mass spectrometry ( UHPLC-MS/MS)/food-borne stimulant drug/residues/beef tissue分类
化学化工引用本文复制引用
齐鹤鸣,韩深,吕美玲,尹志强,严华,李建辉,崔凤云..超高效液相色谱-串联质谱法同时测定牛肉中18种食源性兴奋剂类药物残留[J].色谱,2018,36(7):621-628,8.基金项目
国家质量监督检验检疫总局科技计划项目(2017IK143,2016IK018,2016IK021).Science and Technology Planning Project of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China( Nos.2017IK143,2016IK018,2016IK021). （2017IK143,2016IK018,2016IK021）
