色谱2018，Vol.36Issue(7)：629-633,5.DOI:10.3724/SP.J.1123.2018.01049
稳定同位素稀释超高效液相色谱-串联质谱法测定饲料中氟虫腈及其代谢物
Determination of fipronil and its metabolites in feeds by stable isotope dilution ultra high performance liquid chromatography-tandem mass spectrometry
周鹏 1黄芊 2欧阳立群 2王征 2孟鹏 2戴明 2王瑛2
作者信息
- 1. 南京理工大学化工学院,江苏 南京 210094
- 2. 国家加工食品质量监督检验中心(福州),福建 福州 350002
- 折叠
摘要
Abstract
A method based on ultra high performance liquid chromatography-tandem mass spectrometry( UHPLC-MS/MS)was established for the determination of fipronil and its metab-olites in feeds.Sodium chloride was added to the samples after the ultrasonic extraction of pure water and acetonitrile,and then cleaned up by the QuEChERS method.The target analytes were separated on a Waters HSS T3 C18column using methanol and water as mobile phases with gradient elution, and detected by a MS/MS system under negative electro-spry ionization (ESI-)and the multiple reaction monitoring( MRM)mode.The limits of detection( LODs) were 0.05 μg/kg,and the limits of quantification( LOQs)were 0.2 μg/kg.The linear correla-tion coefficients were more than 0.999 8.The average recoveries ranged from 92.3% to 105.4%with RSDs no more than 2.3%.This method is suitable for the identification of fipronil and its metabolites in feeds because of its simplicity and high sensitivity.关键词
超高效液相色谱-串联质谱/QuEChERS/同位素稀释/氟虫腈及其代谢物/饲料Key words
ultra high performance liquid chromatography-tandem mass spectrometry ( UHPLC-MS/MS)/QuEChERS/isotope dilution/fipronil and its metabolites/feeds分类
化学化工引用本文复制引用
周鹏,黄芊,欧阳立群,王征,孟鹏,戴明,王瑛..稳定同位素稀释超高效液相色谱-串联质谱法测定饲料中氟虫腈及其代谢物[J].色谱,2018,36(7):629-633,5.
