色谱2018，Vol.36Issue(7)：700-704,5.DOI:10.3724/SP.J.1123.2018.01028
超高效液相色谱-串联质谱快速测定食品中的新品种甜味剂爱德万甜
Determination of a new sweetener advantame in food by ultra-high performance liquid chromatography-tandem mass spectrometry
汪辉 1黄小贝 1刘江 1王柯 1葛芬1
作者信息
- 1. 长沙市食品药品检验所,湖南 长沙 410016
- 折叠
摘要
Abstract
A new ultra-high performance liquid chromatography-tandem mass spectrometry with triple quadrupole method was developed,and validated for screening and confirmation of the new sweetener advantame in food.The sample was extracted with methanol-water(50∶50, v/v),followed by centrifugation.The supernatant was then filtered through a membrane filter.The determinant was performed on Agilent SB-C18(150 mm×2.1 mm,5 μm)column with gradient elution.The target compound was carried out by positive electrospray ionization (ESI﹢)under the multiple reaction monitoring(MRM)mode.While the qualitative screening was achieved with retention time and qualitative ion,the confirmation analysis was achieved with peak area and quantitative ion.The results showed that the limit of detection( LOD,S/N≥3)and the limit of quantification( LOQ,S/N≥10)were 0.03 and 0.10 mg/kg,respectively.The average spiked recoveries showed a variation from 80.3 to 98.0% for the three fortification levels.The results showed good linear relationships in the range of 0.01-1.0 mg/L of advan-tame with correlation coefficients above 0.997.This procedure was noticeably rapid,simple, sensitive,and accurate.Therefore,this method is suitable for batch determination of the new sweetener advantame in beverages,yogurt,and jelly.关键词
超高效液相色谱-串联质谱/爱德万甜/新品种甜味剂/食品Key words
ultra-high performance liquid chromatography( UHPLC)-tandem mass spectrome-try/advantame/new sweetener/food分类
化学化工引用本文复制引用
汪辉,黄小贝,刘江,王柯,葛芬..超高效液相色谱-串联质谱快速测定食品中的新品种甜味剂爱德万甜[J].色谱,2018,36(7):700-704,5.
