水生生物学报2018，Vol.42Issue(4)：663-672,10.DOI:10.7541/2018.082
团头鲂GPR43基因克隆、组织分布及黄连素对其mRNA表达量的影响
GENE CLONING OF GPR43 AND ITS DISTRIBUTION IN THE TISSUE OF BLUNT SNOUT BREAM AND THE EFFECT OF BERBERINE ON ITS MRNA EXPRESSION
摘要
Abstract
This study was conducted to explore the effects of berberine on the mRNA expression of G protein coupled receptors 43 (GPR43) and the lipid metabolism performance of blunt snout bream fed with high-fat diet. GPR43 in blunt snout bream was cloned and sequenced with a RACE assays. The effects of berberine on GPR43 mRNA expres-sion in tissues were also investigated. The obtained full-length nucleotide sequence covers 2026 bp, which has an open reading frame of 981 bp, and translates a total of 326 amino acids. Moreover, GPR43 mRNAs were detected in differ-ent tissues of blunt snout bream. In blunt snout bream, the expression of GPR43 in intestine, hepatopancreas, gill and white muscle were the highest. 320 fish (80.00±0.90) g were randomly divided into four trial groups (each has four rep-licates) and fed with 4 diets (normal diet, normal diet with 50 mg/kg berberine, high-fat diet, and high-fat diet with 50 mg/kg berberine, among which normal diet has 5% lipid and high-fat diet has 10% lipid), respectively. The expression of GPR43 mRNA in the high-fat diet group decreased compared with control group, while it significantly increased (P＜0.05) after adding in berberine. Compared with the control group, the contents of free fatty acids (FFA), trigly-cerides (TG), cholesterol (CHO), and mRNA expressions of Cytokinins protein kinase (p38), and Peroxidase value-ad-ded factor (PPARγ) all increased (P＜0.05) in the high-fat group, but a dramatic decrease ( P＜0.05) happened after adding in berberine. The same result was found on the composition of Polyunsaturated fatty acids (PUFA) in hepato-pancreas and muscle tissues. A completely inverse variation was presented on the mRNA expressions of Carnitine palm acyltransferaseⅠ(CPTⅠ), Peroxidase value-added factor (PPARα&β) and AMP dependent protein kinase (AMPK ), as well as on the compositions of Saturated fatty acids (SFA) and Monounsaturated fatty acids (MUFA) in both tissues. Nevertheless, the difference between berberine supplemental normal diet group and control group was out of regular pattern. So further work is still needed to explore the role of berberine in normal circumstances. Overall, the results showed the supplement of berberine could slow down lipid deposition in hepatopancreas, and restore lipid homeostasis which has been damaged by continuous high-fat diet feeding.关键词
黄连素/团头鲂/脂肪代谢性能/G蛋白偶联受体43Key words
Berberine/Megalobrama amblycephala/Lipid metabolism property/G protein coupled receptors 43分类
农业科技引用本文复制引用
陈丹红,陈青青,刘文斌,张定东,徐维娜..团头鲂GPR43基因克隆、组织分布及黄连素对其mRNA表达量的影响[J].水生生物学报,2018,42(4):663-672,10.基金项目
国家自然科学基金面上项目(31472292)资助 [Supported by the NSFC General Program (31472292)] （31472292）
