水生生物学报2018，Vol.42Issue(4)：673-680,8.DOI:10.7541/2018.083
银鲫转录调控因子lbh-b cDNA克隆与表达分析
CLONE AND EXPRESSION ANALYSIS OF TRANSCRIPTION COFACTOR LBH-B in GIBEL CARP
摘要
Abstract
LBH (limb-bud and heart) is a novel high-conserved transcription cofactor in vertebrates involved in em-bryonic development and pathogenesis of some human disease. We screened the lbh-b gene that was abundantly ex-pressed in the pituitary of gibel carp, and cloned the lbh-b (Cglbh-b) cDNA sequence of lbh gene family from gibel carp cDNA library by RACE-PCR. The full-length of Cglbh-b cDNA was 1526 bp with a 549 bp long open reading frame (ORF) coding a 182 amino acid protein. Bioinformatics analysis showed that CgLbh-b protein shared high homology (＞68%) with other vertebrate LBH and is one of intrinsic disordered proteins. Cglbh-b was abundantly expressed in pituitary, telencephalon, ovary and eye of adult gibel carp. The expression of Cglbh-b in female pituitaries was 4.66 times higher than that in male pituitaries, and Cglbh-b in pituitary was up-regulated during the process of oocyte matura-tion. During the early embryonic development, maternal mRNA of Cglbh-b was detected in embryos from 4-cells stage to gastrula stage, and its transcripts were synthesized at bud stage. The results of whole mount in situ hybridization showed that Cglbh-b was distributed on the brain and eyes of embryos from 2 days post fertilization (dpf) to 3dpf. These results suggest that Cglbh-b may play important roles in brain and retina development, and reproductive regula-tion in oocyte maturation of gibel carp.关键词
银鲫/lbh-b/胚胎发育/卵子成熟Key words
Gibel carp/lbh-b/Embryogenesis/Oocyte maturation分类
生物科学引用本文复制引用
李文华,汪洋,李志,桂建芳,周莉..银鲫转录调控因子lbh-b cDNA克隆与表达分析[J].水生生物学报,2018,42(4):673-680,8.基金项目
中国科学院前沿科学重点研究项目(QYZDY-SSW-SMC025) （QYZDY-SSW-SMC025）
现代农业产业技术体系(NYCYTX-49) （NYCYTX-49）
福建省自然科学基金面上项目(2016J01161)资助 [Supported by Key Research Program of Frontier Sciences, CAS (QYZDY-SSW-SMC025) （2016J01161）
the Special Fund of China Agriculture Research System (NYCYTX-49) （NYCYTX-49）
the Natural Science Foundation of Fujian Province, China (2016J01161)] （2016J01161）
