水生生物学报2018，Vol.42Issue(4)：690-697,8.DOI:10.7541/2018.085
七鳃鳗myd88基因的生物学特性及其下游分子的表达模式分析
THE CLONING AND BIOLOGICAL CHARACTERISTICS OF MYD88 IN LAMPREY AND THE EXPRESSION PATTERN OF ITS DOWNSTREAM PROTEINS
摘要
Abstract
Myeloid differentiation factor 88 (MYD88) is a key adapter protein in Toll-like receptor (TLR) signaling, which plays significant role on the innate and adaptive immunity. In this study, a myd88 gene from lamprey (Lampetra japonica) was obtained. The ORF of myd88 was 852 bp in length, encoding a polypeptide of 283 amino acids. The theo-retical molecular weight of lamprey Myd88 was 32.432 kD with a theoretical isoelectric point of 6.25. Lamprey Myd88 was predicted to have no signal peptide. Multiple protein sequence alignment revealed highly conserved death domain in N-terminal, and Box1, Box2, and Box3 in the C-terminal TIR domain, which indicated that lamprey Myd88 was ho-mologous with the MYD88 in other species. Quantitative real-time PCR analysis showed lamprey myd88 gene was exten-sively expressed in all detected tissues. The highest expression level was observed in gill, which was followed by mar-row and kidney. Under in vivo stimulation by lipopolysaccharide (LPS), the expression of myd88 significantly in-creased in leukocytes, followed by gill, which implicated its role in the defense of L. Japonica against bacteria. Further-more, increasing expressions of downstream proteins in Myd88-dependent TLR signaling pathway, including Irak1, Traf6, Ikkβ, and Nfkb, were detected in the tested tissues stimulated with LPS. These results suggested that a con-served Myd88-dependent TLR signaling pathway was found in the lamprey, which play a fundamental role on the ex-ploration of the origin and evolution of the signaling pathway in immune response in future.关键词
七鳃鳗/髓样分化因子88/表达模式/免疫应答Key words
Lamprey/Myeloid differentiation factor 88 (MYD88)/Expression pattern/Immune response分类
生物科学引用本文复制引用
赵春晖,赵培哲,刘欣,李庆伟..七鳃鳗myd88基因的生物学特性及其下游分子的表达模式分析[J].水生生物学报,2018,42(4):690-697,8.基金项目
国家自然科学基金(31170353) （31170353）
辽宁省教育厅重点实验室项目(LZ2015049)资助 [Supported by the National Natural Science Foundation of China (31170353) （LZ2015049）
the Key Laboratory of Liaoning Provincial Education Department (LZ2015049)] （LZ2015049）
