水生生物学报2018，Vol.42Issue(4)：698-708,11.DOI:10.7541/2018.086
饲料中添加雨生红球藻粉对三疣梭子蟹雌体卵巢发育、色泽、抗氧化能力和生化组成的影响
EFFECTS OF DIETARY SUPPLEMENTATION WITH HAEMATOCOCCUS PLUVIALIS POWDER ON OVARIAN DEVELOPMENT, COLORATION, ANTIOXIDANT CAPACITY AND BIOCHEMICAL COMPOSITION OF ADULT FEMALE SWIMMING CRAB, PORTUNUS TRITUBERCULATUS
摘要
Abstract
Swimming crab (Portunus trituberculatus) is an important mariculture crab, and the light color of the pond-reared crabs may be related to low level of dietary astaxanthin. This study was designed to investigate the effects of die-tary astaxanthin on ovarian development, coloration, antioxidant capacity, immune performance and biochemical com-position of female P. trituberculatus. Haematococcus pluvialis powder was used as natural astaxanthin source to formu-late four experimental diets with different astaxanthin levels (0, 26.60, 41.62, and 81.37 mg/kg, defined as Diet 1#—4#). The four experimental diets were fed to female P. trituberculatus for 45 days during the ovarian development;therefore, there were four treatments in this study. The results showed as follows: (1) Dietary astaxanthin had no sig-nificant effects on gonadosomatic index (GSI) and hepatosomatic index (HSI). (2) Total carotenoids content, redness (a*) and yellowness (b*) values of hepatopancreas, ovaries and carapace increased significantly with increasing dietary astaxanthin, while the lightness (L*) values of these tissues showed a decreasing trend (P＜0.05). (3) For antioxidant in-dices, the activities of super oxide dismutase (SOD) and peroxidase (POD) in treatments (Diet 2#—4#) with supple-mentation of astaxanthin were significantly lower than those in Diet 1# treatment without supplementation of asta-xanthin. The activities of total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) in hemolymph as well as the SOD, T-AOC in hepatopancreas increased significantly with elevated dietary astaxanthin, while a decreas-ing trend was found for the malondialdehyde (MDA) content in the hemolymph and hepatopancreas. (4) For the im-mune indices, Diet 4# treatment had the highest activity of acid phosphatase (ACP) in hemolymph and hepatopancreas, while Diet 1# treatment had the significantly higher hemolymph hemocyanin (Hc) content than other treatments (P＜0.05). (5) As for the biochemical composition, the increasing trend was found on the contents of total lipids and total carbohydrate in the muscle and hepatopancreas as well as ovarian lipids with increasing dietary astaxanthin, while Diet 2# treatment had the highest total carbohydrate content in the ovaries among the four treatments (P＜0.05). In con-clusion, dietary supplementation of astaxanthin had no significant effect on ovarian development of P. trituberculatus, but could improve the coloration, antioxidant capacity and the contents of total lipids and total carbohydrate in edible tissues. These results suggest the appropriate level of astaxanthin in fattening diet was approximately 50 mg/kg for adult female P. trituberculatus.关键词
三疣梭子蟹/育肥饲料/虾青素/卵巢发育/抗氧化能力/生化组成Key words
Portunus trituberculatus/Fattening diet/Astaxanthin/Ovarian development/Antioxidant capacity/Biochemical composition分类
农业科技引用本文复制引用
吴仁福,龙晓文,侯文杰,潘桂平,刘建国,成永旭,吴旭干..饲料中添加雨生红球藻粉对三疣梭子蟹雌体卵巢发育、色泽、抗氧化能力和生化组成的影响[J].水生生物学报,2018,42(4):698-708,11.基金项目
上海市科技兴农重点推广项目(沪农科推字(2016)第 1-1-8号) （沪农科推字(2016）
江苏省科技厅苏北科技专项项目(SZ-LYG2017019) （SZ-LYG2017019）
上海市科委工程技术中心能力提升项目(16DZ2281200) （16DZ2281200）
上海高校水产学高峰学科建设项目(2015-62-0908)资助 [Supported by the Extension Project from Shanghai Agriculture Committee (2016, No. 1-1-8) （2015-62-0908）
the Science and Technology Special Project of Northern of Jiangsu Province Department (SZ - LYG2017019) （SZ - LYG2017019）
the Capacity Promotion Projects of Shanghai Engineering and Technology Center from Shanghai Municipal Science and Technology Commission (16DZ2281200) （16DZ2281200）
the Shanghai Universities Top Disciplines Project of Fisheries from Shanghai Municipal Education Committee (2015-62-0908)] （2015-62-0908）
