水生生物学报2018，Vol.42Issue(4)：719-727,9.DOI:10.7541/2018.088
发酵豆粕替代鱼粉和豆粕对罗氏沼虾生长、血清生化及免疫基因表达的影响
THE REPLACEMENT OF FISH MEAL AND SOYBEAN MEAL TO FERMENTED SOYBEAN MEAL AND ITS EFFECTS ON THE GROWTH PERFORMANCE, SERUM BIOCHEMICAL INDICES, AND IMMUNE GENE EXPRESSION IN GIANT RIVER PRAWN, MACROBRACHIUM ROSENBERGII
摘要
Abstract
An experiment was conducted to investigate the effects of replacing fish meal (FM) and soybean meal (SBM) by fermented soybean meal (FSBM) on growth performance, serum biochemical indices, and immune genes in giant river prawn, Macrobrachium rosenbergii, and to find a suitable proportion of FSBM to replace FM in diets. Five isonitrogenous and isoenergetic diets were formulated for giant river prawn, among which a basal diet (T0) containing 30% FM and 18% SBM was treated as the control group, and the FM and SBM (2∶1) in the basal diets replaced by 2% (T2), 5% (T5), 8% (T8) and 15% (T15) of FSBM respectively were treated as experimental diets. Giant river prawns with initial average body weight of (0.17±0.02) g were cultured for 64 days in indoor net cages at a cement tank. Re-sults showed that with increasing FSBM supplemental level, the growth rate and specific growth rate increased firstly and then decreased, and the highest value was found in group T8. Serum superoxide dismutase activity and malondial-dehyde content firstly increased and then decreased. MDA of all experimental groups was higher than that of the con-trol group. Serum aspartate transaminase, alanine aminotransferase activity and total protein content had decreasing trend followed by an increasing trend, and the values in all experimental groups were lower than the control group. Gills Toll receptor mRNA and NF-κB mRNA expression levels and hepatopancreas heat shock protein mRNA relative expression levels in group T15 were the highest among all groups. All these results indicated that the replacement of FM and SBM with FSBM would affect growth, antioxidant ability and immunity, and the optimum amount of FSBM was 8% in the diets in this experiment.关键词
罗氏沼虾/鱼粉/豆粕/发酵豆粕/血清生化/免疫基因Key words
Macrobrachium rosenbergii/Soybean meal/Fermented soybean meal/Fish meal/Serum biochemical indices/Immune genes分类
农业科技引用本文复制引用
杨景丰,华雪铭,郭子好,刘韬,孔纯,冯悦,王刚..发酵豆粕替代鱼粉和豆粕对罗氏沼虾生长、血清生化及免疫基因表达的影响[J].水生生物学报,2018,42(4):719-727,9.基金项目
上海市科委高校能力建设项目(14320502000) （14320502000）
农业部淡水水产种质资源重点实验室开放课题资助 [Supported by Shanghai Science and Technology Committee (14320502000) （14320502000）
Key Laboratory of Freshwater Aquatic Genetic Resources] （）
