水生生物学报2018，Vol.42Issue(4)：728-735,8.DOI:10.7541/2018.089
饲料中添加谷胱甘肽对黄颡鱼幼鱼组织谷胱甘肽含量、免疫及抗氧化性能的影响
EFFECTS OF DIETARY SUPPLEMENTATION WITH GLUTATHIONE ON TISSUE GSH LEVEL, IMMUNITY, AND ANTIOXIDANT CAPACITY OF JUVENILE YELLOW CATFISH ( PELTEOBAGURS FULVIDRACO)
摘要
Abstract
To assess the effects of dietary supplementation with glutathione (GSH) on tissue GSH levels, immunity, and antioxidant capacity of juvenile yellow catfish (Pelteobagrus fulvidraco), a total of 800 yellow catfish with a mean body weight of (1.32±0.01) g were considered and randomly divided into 5 groups with 4 replicates per each group and 40 fish per each replicate as well. During 56d, the fish were fed by a basal diet and four test diets, which supplemented with 100, 300, 500, and 700 mg/kg of reduced GSH, respectively. The results indicated that, except for 100 mg/kg group, supplementation with GSH significantly increased the GSH content in liver and serum of yellow catfish (P＜0.05). When GSH level was 300 mg/kg or higher, GSH content in liver and serum was in a stable state. With the in-crease of glutathione level, the immunity and antioxidant indices were initially increased, and then decreased. The lyso-zyme and alkaline phosphatase activities in 300 and 500 mg/kg groups, immunoglobulin M (IgM) and complement component 4 (C4) contents in 300 mg/kg group, and acid phosphatase activity in 500 mg/kg group markedly increased, compared with the control group (P＜0.05). There were no significant differences (P＞0.05) in complement component 3 (C3) and nitric oxide contents among all groups. Compared with the control and 700 mg/kg groups, the liver supero-xide dismutase, catalase and glutathione peroxidase activities, total antioxidant capacity and the serum superoxide dis-mutase, as well as glutathione peroxidase activities in 300 mg/kg group were significantly higher (P＜0.05), while the serum malondialdehyde content in that group was considerably lower (P＜0.05). Under ammonia-nitrogen stress for 96h, compared with the control group, the liver and serum superoxide dismutase, catalase, and glutathione peroxidase activities in 300 mg/kg group were significantly greater (P＜0.05), however, the serum malondialdehyde content in the mentioned group was significantly lower (P＜0.05). In conclusion, dietary supplementation with GSH could enhance the tissue GSH level, immunity and antioxidant capacity of juvenile yellow catfish, and the effective range of this enhance-ment was 300 to 500 mg/kg as well.关键词
谷胱甘肽/黄颡鱼/GSH含量/免疫/抗氧化/氨氮应激Key words
Glutathione/Yellow catfish (Pelteobagrus fulvidraco)/GSH content/Immune/Antioxidation/Ammonia-nitrogen stress分类
农业科技引用本文复制引用
周艳玲,孙育平,黄燕华,王国霞,陈冰,王卫民,曹俊明,易昌金..饲料中添加谷胱甘肽对黄颡鱼幼鱼组织谷胱甘肽含量、免疫及抗氧化性能的影响[J].水生生物学报,2018,42(4):728-735,8.基金项目
广东省现代农业科技创新联盟建设(2017LM082和2017LM083) （2017LM082和2017LM083）
2017年广东省渔业厅海洋和渔业发展专项资金(科技攻关与研发)资助 [Supported by the Construction of Modern Agricultural Science and Technology Innovation Alliance in Guangdong Province (2017LM082 and 2017LM083) （科技攻关与研发）
Special Fund for Marine and Fishery Development of Guangdong Fisheries Department in 2017 (Research and Development of Science and Technology)] （Research and Development of Science and Technology）
