生物骨科材料与临床研究2018，Vol.15Issue(3)：6-9,后插2,5.DOI:10.3969/j.issn.1672-5972.2018.03.002
hMSCs与同种异体骨共培养及其在促红素诱导下的成血管效应
The angiogenesis promoting effect of erythropoietin on hMSCs co-cultured with allogeneic bone scaffold
摘要
Abstract
Objective To investigate the proliferation and erythropoietin(EPO)-induced angiogenesis of human mesenchymal stem cells (hMSCs) co-cultured with allogeneic bone scaffold. Methods The experiment was finished in the laboratory of Xinqiao hospital affiliated to the third military medical university. ①The hMSCs cultured in vitro were divided into two groups: the normal group and the experimental group. In experimental group, the hMSCs were cocultured with allogeneic bone scaffold. CCK-8 (Cell Counting Kit-8 reagent) was used to confirm hMSCs proliferation on days 1, 2, 3, and 4 d. ②The hMSCs were induced in three groups: group A (hMSCs+ EPO + allogeneic bone), group B (hMSCs+ EPO), group C (hMSCs + allogeneic bone). Induced cells were characterized by immunocytochemistry platelet endothelial cell adhesion molecule 1 (PECAM-1), von willebrand factor (VWF), and vascular endothelial growth factor (VEGF). The number of induced cells was measured by using image analysis software. Results After co-cultured with allogeneic bone scaffold, there was no significant difference on hMSCs proliferation rate between the experimental group and the normal group (P>0. 05). The significant expressions of PECAM-1, VEGF and VWF were observed in group A and group B, but there was no significant statistical difference between group A and group B(P>0. 05). The expressions of PECAM-1, VEGF and VWF in group C were not distinct. Conclusion The hMSCs co-cultured with allogeneic bone scaffold can proliferate well and can be differentiated into endothelial cells by EPO.关键词
间充质干细胞/促红细胞生成素/同种异体骨/骨坏死/治疗Key words
Mesenchymal stem cells/Erythropoietin/Allogeneic bone/Osteonecrosis/Therapy分类
医药卫生引用本文复制引用
万岷,金相廷,张幸,黄俊,陈桂林,夏红枝,李东洋,卢振刚,刘希娥..hMSCs与同种异体骨共培养及其在促红素诱导下的成血管效应[J].生物骨科材料与临床研究,2018,15(3):6-9,后插2,5.基金项目
湛江市科技计划项目(2013A01016) （2013A01016）
