天津中医药2018，Vol.35Issue(7)：521-525,5.DOI:10.11656/j.issn.1672-1519.2018.07.13
扶肾方对尿毒症大鼠腹膜间充质TGF－β1、E－cadherin和α－SMA表达的影响
Effects of Fushen recipe on expression of TGF-β1, E-cadherin and α-SMA in peritoneal mesenchymal membranes of uremia rats
摘要
Abstract
[Objective] To invigorate the kidney in uremic rats TGF-β1, expression of E-cadherin and α-SMA. [Methods] SD rats underwent 5/6+1.5% renal dialysis Baxter replication in rat model of peritoneal fibrosis. After 6 weeks'intervention, the serum levels of creatinine and urea nitrogen were detected. The pathological changes of peritoneum were observed by HE staining. The expression of E-cadherin and α-SMA in peritoneal mesothelial markers in each group was detected by RT-PCR method, and the content of TGF-β1 was detected by ELISA. [Results] The results help kidney prescription intervention, serum creatinine and urea nitrogen were decreased, especially in the high dose group was most significant (P＜0.05); Fushen recipe Group of peritoneal mesothelial cell degeneration, shedding, inflammatory cell infiltration and neovascularization were obviously reduced; the results of RT-PCR showed that E-cadherin decreased in model group compared with normal group the expression, the difference was statistically significant (P＜0.05); TGF-β1 and α-SMA compared with normal group expression, the difference was statistically significant (P＜0.01), compared with the model group, the intervention group to help kidney. The expression of E-cadherin was increased, and the high dose group increased most significantly (P＜0.01); TGF-β1 and α-SMA in middle and high dose groups had statistically significant difference (P＜0.05, P＜0.01); the other group compared with control group, low dose group decreased, the results were not statistically significant and medium and high dose group had statistically significance (P＜0.05). [Conclusion] Fushen recipe may inhibit the occurrence of PMCs EMT by inhibiting TGF-β1 and regulating the expression of E-cadherin and α-SMA.关键词
扶肾方/腹膜纤维化/腹膜透析Key words
Fushen recipe/peritoneal fibrosis/peritoneal dialysis分类
医药卫生引用本文复制引用
雷洋洋,杨波,裴明,林燕,杨洪涛..扶肾方对尿毒症大鼠腹膜间充质TGF－β1、E－cadherin和α－SMA表达的影响[J].天津中医药,2018,35(7):521-525,5.基金项目
国家自然科学基金项目(81704007,81303092, 81774065) （81704007,81303092, 81774065）
天津市科技计划项目(15ZXLCSY00020). （15ZXLCSY00020）
