土壤学报2018，Vol.55Issue(3)：744-753,10.DOI:10.11766/trxb201711060470
稻田土壤厌氧氨氧化菌群落结构对长期不同施肥的响应
Response of Paddy Soil Anammox Bacteria to Long-Term Fertilization in Community Structure
摘要
Abstract
[Objective] This study was conducted to investigate abundance and community structure of anaerobic ammonia oxidizing (anammox) bacteria in paddy soils under long-term fertilization and explore mechanism of anammox bacteria responding to different fertilizers, in an attempt to provide a scientific basis for rationalizing fertilization and understanding ammonia oxidizing processes in wetland ecosystems. [Method] Four treatments, i.e. control (CK), chemical fertilizer (NPK), chemical fertilizer plus cattle manure (NPKM), and chemical fertilizer plus straw (NPKS), were set in the field experiment of the study. Soil samples were collected from the treatments for analysis of abundance and community structure of soil anammox bacteria, using the fluorescent quantitative PCR assay targeting hydrazine synthase α-subunit (hzsA) gene and high-throughput sequencing technology aiming at 16S rRNA gene of anammox bacteria.[Result]Significant differences were observed between the treatments in abundance of hzs A gene (p<0.05), exhibiting an order of NPKM>NPKS>NPK>CK. Correlation analysis ( p<0.05) shows that abundance of hzs A gene was significantly and positively related to soil organic matter, total nitrogen and ammonia content. High-throughput sequencing shows Candidatus Brocadia, Candidatus Anammoxoglobus and Candidatus Scalindua were the main anammox bacteria and Candidatus Brocadia the dominant one in all the treatments. Bacteria diversity analysis shows that treatments CK and NPKS were significantly higher than treatment NPKM and NPK in Shannon index, Simpson index and Chao 1 index (p<0.05). [Conclusion] Long-term fertilization alters the anammox bacteria community in population and structure. The application of chemical fertilizers combined with organic manure is conducive to abundance of the anammox bacteria. However, the anammox bacteria community in the soil varies in structure with difference in fertilization. The anammox bacteria community increased in α-diversity in the soil applied with chemical fertilizer plus straw, but decreased in the soil applied with chemical fertilizer only and chemical fertilizer plus cattle manure. It can, therefore, be concluded that anammox bacteria vary in their response to different fertilizer or manure used in abundance and community structure.关键词
厌氧氨氧化菌/长期施肥/高通量测序/荧光定量PCR/ Candidatus BrocadiaKey words
Anammox bacteria/ Long-term fertilization/ High-throughput sequencing/ q-PCR/Candidatus Brocadia分类
农业科技引用本文复制引用
聂三安,王祎,王飞,杨静,周碧青,邢世和..稻田土壤厌氧氨氧化菌群落结构对长期不同施肥的响应[J].土壤学报,2018,55(3):744-753,10.基金项目
国家自然科学基金项目(41671490,4170010194)、福建省自然科学基金项目(2017J0101612)和福建农林大学国际合作项目(KXB16016A)共同资助Supported by the National Natural Science Foundation of China (Nos. 41671490 and 4170010194),the Natural Science Foundation of Fujian Province, China (No. 2017J0101612) and the International Cooperation Project of Fujian Agriculture and Forestry University (No. KXB16016A) （41671490,4170010194）
