西安交通大学学报（医学版）2018，Vol.39Issue(4)：514-518,5.DOI:10.7652/jdyxb201804014
水溶性辅酶Q10对鱼藤酮致PC12细胞凋亡的保护作用及其机制
Protective effect of water soluble CoQ10 on rotenone-induced apoptosis in PC12 cells
摘要
Abstract
Objective To investigate the protective effect and the underlying mechanism of water soluble coenzyme Q10 (CoQ10)against rotenone induced injury on PC12 cells model.Methods PC12 cells were cultured with rotenone,water-soluble CoQ1 0 was added to the culture media 3 hours prior to the rotenone incubation.We determined cell viability by CCK8;reactive oxygen species (ROS)was detected by spectrophotometer;and Bcl-2, Bax,active Caspase-3,Caspase-9 and apoptosis-inducing factor (AIF)were measured by Western blotting after 24-hour rotenone incubation.Results After the treatment by rotenone,cell viability decreased significantly (P＜0.01)and ROS level increased (P＜0.01).CoQ10 could improve PC12 cell viability (P＜0.01)and reduce the level of ROS (P＜0.01).Western blotting experiments showed that CoQ10 could reduce rotenone-induced Caspase-9 (P＜0.05),active Caspase-3 (P＜0.05)and Bax (P＜0.01)expressions,increase the expression of Bcl-2 (P＜0.01),and prevent nuclear translocation of AIF (P＜0.05).Conclusion CoQ10 has a protective effect on rotenone-induced apoptosis in PC12 cells,the mechanism of which may be through scavenging ROS in cells;decreasing caspase-9 ,active caspase-3 and Bax expressions;and increasing the expression of Bcl-2 ;and preventing AIF nuclear translocation.关键词
水溶性辅酶Q10/鱼藤酮/帕金森病/PC12细胞Key words
water soluble coenzyme Q10/rotenone/Parkinson's disease/PC12 cell分类
医药卫生引用本文复制引用
江毓敏,李海宁,林邵青,陈燕燕,安静,马春换,宦楠楠,成江..水溶性辅酶Q10对鱼藤酮致PC12细胞凋亡的保护作用及其机制[J].西安交通大学学报（医学版）,2018,39(4):514-518,5.基金项目
国家自然科学基金资助项目(No.81460179).Supported by the National Natural Science Foundation of China (No.81460179) （No.81460179）
