陕西科技大学学报2018，Vol.36Issue(4)：42-46,5.
β-环糊精对黄曲霉毒素B1的荧光增敏效应研究
The research of fluorescence enhancement effect of aflatoxin B1 by β-cyclodextrin
摘要
Abstract
The effect of β-cyclodextrin(β-CD)concentration,metal ions,aliphatic alcohols,temperature,pH and ultrasonic time on the aflatoxin B1(AFB1)fluorescence enhanced by β-CD were studied.In addition,the inclusion characteristics of AFB1-β-CD were analyzed by Benesi-Hildebrand method and thermodynamics method.Results showed that increasing the β-CD concentration,adding Hg2+and isopropyl alcohol could promote the fluorescence en-hancement effect of AFB1by β-cyclodextrin,the low temperature and acid system were favor to inclusion reaction ;However,the ultrasonic time had little effect on the inclusion reaction.The reaction is exothermic and spontaneous,inclusion had the best effect at 25 ℃,the inclu-sion ratio is 1:1,the inclusion constant K＝3.68×107L/mol,Δ S＝-0.11 kJ/mol,Δ H＝-74.03 kJ/mol,Δ G＝-42.38 kJ/mol.关键词
β-环糊精/黄曲霉毒素B1/荧光增强/包合特性Key words
β-cyclodextrin/aflatoxin B1/fluorescence enhancement/inclusion characteristics分类
医药卫生引用本文复制引用
易建华,宁建琴,宋宏新,郭芮,徐丹,吴丹丹,卫梦绮,李亚俊..β-环糊精对黄曲霉毒素B1的荧光增敏效应研究[J].陕西科技大学学报,2018,36(4):42-46,5.基金项目
陕西省科技厅农业科技创新与攻关计划项目(2015YN002) （2015YN002）
陕西科技大学博士科研启动基金项目(BJ12-19) （BJ12-19）
