陕西科技大学学报2018，Vol.36Issue(4)：57-62,6.
基于全基因组Den ovo测序的异常汉逊酵母菌株792基因组重复序列的分布特征研究
Study on distribution characteristics of repeat sequences in the genome of Hansenula anomala 792 based on de novo sequencing
摘要
Abstract
Repeat sequence is an important part of the genome and plays an important role in the evolution of organisms,genetics,and regulation of gene expression.Here,bioinformatics methods were used to study the distribution characteristics of repeat sequences in the genome of Hansenula anomala 792 based on de novo sequencing.The results showed that the total length of the repeat sequences in the whole genome was 346 417 bp,in which the tandem re-peats were 238 264 bp,interspersed repeats were 108 253 bp,accounting for 2.52％of the to-tal genome length,and there was one repeat sequence per 4.05 Kb.The copy number of re-peat units in microsatellite DNA sequences was mostly less than 15 times,the dominant base type was trinucleotide repeats,and the largest number of repeat unit motif was AAC ;the copy number of repeat units in minisatellite DNA was less than that in microsatellite DNA and it mainly distributed from 1 to 3 times,the number of minisatellite DNA with repeat unit greater than 15 bp is decreasing with the increase of repeat unit length.The number of long-terminal repeats(LTRs)was the largest in interspersed repeats,and the average length of rolling coils(RCs)was the longest.Our results will provide a theoretical basis for the molec-ular breeding and developing of SSR molecular markers of H ansenula anom ala.关键词
异常汉逊酵母菌/基因组/小卫星DNA/微卫星DNA/散在重复序列Key words
Hansenula anomala/genome/minisatellite DNA/microsatellite DNA/inter-spersed repeats分类
生物科学引用本文复制引用
王婷,张寒玉,蔡长龙,唐朝,毛培宏,钱卫东,李永东..基于全基因组Den ovo测序的异常汉逊酵母菌株792基因组重复序列的分布特征研究[J].陕西科技大学学报,2018,36(4):57-62,6.基金项目
国家自然科学基金项目(11575149,31760016) （11575149,31760016）
西安市未央区科技计划项目(201707) （201707）
宁波市自然科学基金项目(2017A610268) （2017A610268）
陕西科技大学博士科研启动基金项目(2017BJ-48) （2017BJ-48）
