陕西科技大学学报2018，Vol.36Issue(4)：71-75,81,6.
基于8-羟基久洛尼定的荧光探针用于谷胱甘肽的特异性检测研究
Specific detection of glutathione with a fluorescent probe based on 8-hydroxyjulolidine skeleton
摘要
Abstract
Reduced glutathione(GSH)is a small molecule peptide consisting of three amino acids,w hich is an important active molecule in cells and tissues,associated with a variety of physiological and pathological functions.In this paper,a GSH fluorescent probe based on coumarin fluorophore was designed and synthesized by using 8-hydroxyjulolidine skeleton.The planarity of the fluorophore was enhanced by the rigid planar structure of 8-hydroxyjulo-lidine,which achieves a red shift of the fluorescence wavelength.The spectral analysis indi-cates that the fluorescence probe has a high specific response to GSH.The gradual decrease of the fluorescence at 516 nm and increase at 561 nm reveal a typical ratiometric response to GSH.Meanwhile,the good linear relation between the fluorescent intensity and concentration of GSH indicates that the probe can be used for quantitative detection of GSH.关键词
还原型谷胱甘肽/荧光探针/8-羟基久洛尼定/香豆素/比率Key words
reduced glutathione/fluorescent probe/8-hydroxyjulolidine/coumarin/ratiomet-ric分类
化学化工引用本文复制引用
王超,闫钰佳,张诣为,武雨珽,代方方..基于8-羟基久洛尼定的荧光探针用于谷胱甘肽的特异性检测研究[J].陕西科技大学学报,2018,36(4):71-75,81,6.基金项目
国家自然科学基金项目(21708023) （21708023）
陕西科技大学博士科研启动基金项目(2017GBJ-04) （2017GBJ-04）
