中国组织工程研究2018，Vol.22Issue(16)：2461-2466,6.DOI:10.3969/j.issn.2095-4344.0275
补肾健脾活血方含药血清提高过表达Sost转染成骨细胞增殖和碱性磷酸酶活性
Bushen Jianpi Huoxue decoction increases the proliferation of Sost-overexpressed adenovirus transfected osteoblasts and alkaline phosphatase activity
摘要
Abstract
BACKGROUND: Chinese herbs have been shown to affect osteoblasts through Wnt/β-catenin signaling pathway. Sclerostin (Sost) is an inhibitor of Wnt signaling pathway and an inhibitor of osteogenesis, and it is also an antagonistic factor that regulates the differentiation and proliferation of osteoblasts. Bushen Jianpi Huoxue decoction (BJHD) is the empirical formula in the prevention and treatment of osteoporosis at the Affiliated Orthopedics Hospital, Guangzhou University of Chinese Medicine. BJHD can promote osteogenesis and effectively treat osteoporosis. OBJECTIVE: To observe the effect of BJHD on the cell proliferation, alkaline phosphatase activity and expression levels of related proteins in Sost-overexpressed adenovirus transfected osteoblasts. METHODS: The osteoblasts were divided into two groups: blank control group (empty adenovirus transfection) and Sost overexpressed group (Sost overexpression and adenovirus transfection). Two groups were cultured with blank serum and medicine serum. The cell proliferation was tested by cell counting kit-8 assay, and alkaline phosphatase activity was tested by alkaline phosphatase kit. Expression levels of bone regulation-related proteins were tested by western blot assay. RESULTS AND CONCLUSION: Compared with the blank serum, medicine serum could increase the cell viability at 24, 48 and 72 hours in both groups (P ＜ 0.01). Compared with the blank serum, medicine serum increased the alkaline phosphatase activity (P ＜ 0.01). Compared with the blank serum, medicine serum in the blank control group decreased the expression of low-density lipoprotein receptor-related protein 5 and osteoprotegerin (P ＜ 0.01), but showed no effect on the expression of osteopontin and tumor necrosis factor α. Medicine serum in the Sost-overexpressed group decreased the expression of low-density lipoprotein receptor-related protein 5, osteoprotegerin and osteopontin, increased the expression of tumor necrosis factor α (P ＜ 0.01). These results indicate that BJHD can increase the proliferation of recombinant adenovirus transfected Sost-overexpressed osteoblasts and alkaline phosphatase activity, and regulate the expression of low-density lipoprotein receptor-related protein 5, osteoprotegerin, osteopontin, and tumor necrosis factor α.关键词
骨质疏松/Sost/过表达基因/细胞实验/补肾健脾活血方分类
医药卫生引用本文复制引用
万雷,黄宏兴,黄红,王凡,柴爽,王吉利,刘少津,黄佳纯..补肾健脾活血方含药血清提高过表达Sost转染成骨细胞增殖和碱性磷酸酶活性[J].中国组织工程研究,2018,22(16):2461-2466,6.基金项目
国家自然科学基金课题(81673786,81302991) （81673786,81302991）
广东省自然科学基金课题(S2013040016828) （S2013040016828）
广东省科技计划项目(2016A020216024)the National Natural Science Foundation of China,No.81673786 and 81302991 （2016A020216024）
the Natural Science Foundation of Guangdong Province,No.S2013040016828 （）
the Science and Technology Program of Guangdong Province,No.2016A020216024 （）
