中国组织工程研究2018，Vol.22Issue(16)：2502-2507,6.DOI:10.3969/j.issn.2095-4344.0219
运动性骨骼肌损伤大鼠膜修复蛋白dysferlin的作用机制
Mechanism by which dysferlin promotes repair of exercise-induced skeletal muscie injury in rats
摘要
Abstract
BACKGROUND: Numerous studies concerning dysferlin focus on muscle diseases (such as muscular dystrophy), but the relationship between membrane repair after exercise-induced muscle damage and dysferlin is little reported. OBJECTIVE: To observe the cell membrane permeability and expression levels of dysferlin and calpain3 in the rat gastrocnemius after acute eccentric exercise, so as to provide an theoretical reference for exploring the molecular mechanism of muscle regeneration and the exercise therapy of muscular diseases. METHODS: Thirty-two Sprague-Dawley rats were randomly divided into four groups including control, 24, 48 and 72 hours post exercise groups. The membrane permeability and expression levels of dysferlin and calpain3 were determined by immunohistochemistry, western blot assay and qRT-PCR. RESULTS AND CONCLUSION: The activity of serum creatine kinase, and expression levels of calpain3 mRNA and dysferlin protein, as well as membrane permeability at 24 hours post exercise were significantly greater than those in the control group (P ＜ 0.05 or 0.01). The expression level of dysferlin mRNA at 24 and 48 hours post exercise was significantly higher than that at 72 hours post exercise (P ＜ 0.05). Therefore, the damage to the skeletal muscle cell membrane was the most severe at 24 hours after eccentric exercise. Due to Ca2+influx, expression of calpain3 mRNA was activated, and then the damaged cell membrane was repaired by increasing the expression of dysferlin.关键词
运动性肌损伤/离心运动/肌酸激酶/Dysferlin/Calpain3/延迟性肌肉酸痛/SNARE复合体/免疫组织化学染色/Western blot/RT-qPCR/组织工程分类
医药卫生引用本文复制引用
黄巧婷,许杰,林家仕..运动性骨骼肌损伤大鼠膜修复蛋白dysferlin的作用机制[J].中国组织工程研究,2018,22(16):2502-2507,6.基金项目
国家自然科学基金(31401017) （31401017）
成都大学自然基金(2017XJR12) （2017XJR12）
中央高校基本科研业务费专项资金(2015BS004)the National Natural Science Foundation of China,No.31401017 （2015BS004）
the Natural Science Foundation of Chengdu University,No.2017XJR12 （）
the Fundamental Research Funds for the Central Universities,No.2015BS004 （）
