中国组织工程研究2018，Vol.22Issue(16)：2537-2541,5.DOI:10.3969/j.issn.2095-4344.0220
运动训练对背根神经节切除模型大鼠运动功能的影响
Effect of treadmill training on the locomotor function in a rat model of dorsal root ganglion resection
摘要
Abstract
BACKGROUND: Peripheral nerve injury can lead to extensive changes in central nervous system, and exercise training can promote the recovery of locomotor function following central nervous system injury. OBJECTIVE: To observe the changes of locomotor function and the expression levels of vesicular glutamate transporter VGLUT1 in the spinal cord in a rat model of dorsal root ganglion resection after treadmill exercise and to explore the effect of treadmill training on the locomotor function after peripheral nerve injury. METHODS: Thirty-nine 10-week-old male Wistar rats were randomized into experimental (n=15), control (n=15) and sham operation (n=9) groups. The rats in the experimental and control groups received the dorsal root ganglion resection at L3and L4segments to establish the model of peripheral nerve injury under local anesthesia, while the rats in the sham operation group were only subjected to dorsal root ganglion exposure. The rats in the experimental group underwent 15 m/minute treadmill training at postoperative 7 days, while rats in the other two groups were in free movement. Gait analysis was performed at preoperative 3 days, postoperative 7, 14, 21, and 28 days, respectively, and the behavioral changes of rats were observed. The tissue sections were obtained from L3segment at postoperative 7, 14, and 28 days to detect the expression levels of VGLUT1 in the spinal cord by immunohistochemistry. RESULTS AND CONCLUSION: The peroneal nerve function index in the experimental and control groups was lower than that before surgery and that in the sham operation group at postoperative different time points (all P ＜ 0.05). The index in the experimental and control groups was the lowest on day 7 postoperatively (P ＜ 0.05), then the index gradually increased, but was still lower than the preoperative level (P ＜ 0.05). The index in the experimental group was significantly higher than that in the control group at postoperative 21 and 28 days (P ＜ 0.05). The expression levels of VGLUT1 in the lamina IX in the experimental and control groups were significantly lower than those in the sham operation group at different time points after surgery (P ＜ 0.01). The levels in the experimental group were significantly higher than those in the control group at postoperative 14 and 28 days (P ＜ 0.05). The levels in both groups on a decline after surgery, especially the control group (P ＜ 0.05). These results suggest that treadmill can promote the recovery of locomotor function post peripheral nerve injury.关键词
脊神经后根神经节切除术/运动训练/步态分析/囊泡谷氨酸转运蛋白 /周围神经损伤/运动康复/wistar大鼠/腓神经功能指数分类
医药卫生引用本文复制引用
安普天,朱文文,李脉超,崔晓娟,周燕,张益萌,金利新..运动训练对背根神经节切除模型大鼠运动功能的影响[J].中国组织工程研究,2018,22(16):2537-2541,5.基金项目
青岛大学医学院国家级实验教学示范中心创新实验项目(201511065055)the Innovative Experiment Project of National Experimental Teaching Demonstration Center of Medical College of Qingdao University,No.201511065055 （201511065055）
