水解南珠液能促进人微血管内皮细胞的增殖和迁移
Hydrolysis of Nanzhu fluid promotes proliferation and migration of human microvascular endothelial cells
摘要
Abstract
BACKGROUND: Pearl has the tranquilizing effect, and it can be applied in the treatment of hypertension. However, there is little report on the prevention and cure effect and mechanism of hydrolyzed pearl liquid on hypertension. OBJECTIVE: To observe the effect of hydrolysis of Hepu pearl (hydrolyzed Nanzhu fluid) on the biological behavior and secretion of human microvascular endothelial cells. METHODS: Human microvascular endothelial cells were cultured and passaged. There were four groups, and the microvascular endothelial cells were incubated in the 200 μL culture medium containing nothing (control group), and 120, 60 and 30 mg/L hydrolysis of Nanzhu fluid. The cell proliferation and migration was detected by cell conuting kit-8 assay and Transwell assay respectively; the cell cycle distribution was tested by flow cytometry; the cell apoptosis was assayed by TUNEL method; the secretion of nitric oxide and reactive oxygen species was tested by nitrale reduetase and chemical fluorescence method, respectively. RESULTS AND CONCLUSION: Compared with the control group, hydrolysis of Nanzhu fluid significantly promoted the proliferation of microvascular endothelial cells in a manner-dependent manner (P ＜ 0.05), suggesting the optimal concentration was 120 mg/L. Compared with the control group, the percentage of cells in S and G2 phase was significantly increased, and the percentage of cells in the G1 phase was significantly reduced in the hydrolysis of Nanzhu fluid group (P＜0.05), indicating hydrolysis of Nanzhu fluid could promote cell cycle progression. Apoptotic cells with green-stained nucleus were invisible in both groups. The number of cell migration in the hydrolysis of Nanzhu fluid group was significantly more than that in the control group (P ＜ 0.05). Compared with the control group, there was a significant increase in the nitric oxide secretion, and a significant decrease in the production of reactive oxygen species in the hydrolysis of Nanzhu fluid group (P＜0.05). To conclude, hydrolysis of Nanzhu fluid can promote the proliferation and migration of human microvascular endothelial cells in vitro, and has the function of promoting the secretion of nitric oxide and inhibiting reactive oxygen species secretion, implying its positive role in the protection of endothelial cell function.关键词
合浦珍珠/南珠/水解/人微血管内皮细胞/增殖/迁移/TUNEL法/Transwell法/生物学行为分类
医药卫生引用本文复制引用
岑妍慧,林湧,林江,贾微,赵静..水解南珠液能促进人微血管内皮细胞的增殖和迁移[J].中国组织工程研究,2018,22(16):2564-2569,6.基金项目
国家级海洋经济创新发展示范城市(北海市)产业链协调创新项目(Bhsfs002) （北海市）
广西自然科学基金重点项目(2015GXNSFDA227002):合浦珍珠"解毒生肌"药效机制及物质基础研究 （2015GXNSFDA227002）
广西科技基地和人才专项(桂科 AD17195080) （桂科 AD17195080）
"北部湾海洋医药研究与开发"广西特聘专家岗位 （）
中国博士后科学基金(2017M623297XB) （2017M623297XB）
广西中医药大学岐黄工程高层次人才团队培育项目(2018001)the National Industrial Chain Coordination Innovation Project of Marine Economy Innovation and Development Model City (Beihai City),No.Bhsfs002 （2018001）
the Key Project of Natural Science Foundation of Guangxi Zhuang Autonomous Region,No.2015GXNSFDA227002 （）
the Science and Technology Base and Talent Specialization of Guangxi Zhuang Autonomous Region,No.AD17195080 （）
Guangxi Specialist Posts of "Beibu Gulf Marine Medicine Research and Development" （）
the National Postdoctoral Science Foundation of China,No.2017M623297XB （）
Qihuang Engineering High-Level Talent Team Cultivation Project of Guangxi University of Chinese Medicine,No.2018001 （）
