中国组织工程研究2018，Vol.22Issue(17)：2625-2630,6.DOI:10.3969/j.issn.2095-4344.0484
小鼠骨髓间充质干细胞成骨分化中的核受体Rev-erbα及Rorα
Roles of Rev-erb alphya and Ror alpha in osteoblastogenesis of mouse bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: The orphan nuclear receptors Rev-erbα and Rorα play important roles in lipid metabolism and glucose metabolism. But it is unclear whether they are involved in bone metabolism. OBJECTIVE: To observe the expression of Rev-erbα and Rorα during the osteoblastogenesis process of bone marrow mesenchymal stem cells (BMSCs). METHODS: BMSCs were isolated and purified from C57BL/6 mice by the whole bone marrow adherence method followed by morphology observations and then BMSCs were induced to differentiate into osteoblasts and adipocytes. We detected their differentiation abilities using oil red O staining and alizarin red staining, respectively. Real-time PCR and western blot assay were conducted to detect the mRNA and protein levels of Rev-erbα and Rorα in BMSCs cultured in the osteogenic medium for 0, 7, 14 days. RESULTS AND CONCLUSION: BMSCs from C57BL/6 mice were mainly spindle-shaped and exhibited the swirl-like pattern of growth. Following induction, oil red O staining and alizarin red staining produced a positive reaction in these cells. Rev-erbα expression at both gene and protein levels decreased at 0, 7, 14 days after osteogenic induction, while Rorα expression increased at both gene and protein levels. These findings indicate that Rev-erbα and Rorα may participate in the osteoblastogenesis of BMSCs.关键词
干细胞/骨髓间充质干细胞/成骨分化/核受体/Rev-erbα/Rorα分类
医药卫生引用本文复制引用
林富伟,徐晓梅,崔琰,谢乙加,赵青..小鼠骨髓间充质干细胞成骨分化中的核受体Rev-erbα及Rorα[J].中国组织工程研究,2018,22(17):2625-2630,6.基金项目
泸州市科技局基金资助计划(2015-S-50(2/3)) （2015-S-50(2/3）
西南医科大学青年基金(2014QN-052) （2014QN-052）
西南医科大学附属口腔医院院级课题(201402) the Fund of Luzhou Municipal Department of Science and Technology, No. 2015-S-50(2/3) （201402）
the Youth Foundation of Southwest Medical University, No. 2014QN-052, the Funded Project by the Affiliated Stomatological Hospital of Southwest Medical University, No. 201402 （）
