中国组织工程研究2018，Vol.22Issue(17)：2631-2636,6.DOI:10.3969/j.issn.2095-4344.0512
体外诱导骨髓间充质干细胞定向分化为软骨细胞:转化生长因子β1、胰岛素样生长因子1的协同刺激作用
Optimization of the protocols for induction and differentiation of bone marrow mesenchymal stem cells into chondrocytes: a synergistic action between transforming growth factor beta1 and insulin-like growth factor 1
谭荣邦 1陈宏明 1罗世官 1李日著 1曾德创1
作者信息
- 1. 右江民族医学院附属医院心胸血管外科,广西壮族自治区百色市 533000
- 折叠
摘要
Abstract
BACKGROUND: Inducing factors are currently used as a main method for the differentiation of bone marrow mesenchymal stem cells (BMSCs) into chondrocytes. OBJECTIVE: To investigate the collaborative stimulation of transforming growth factor beta1 (TGF-β1) and insulin-like growth factor 1 (IGF-1) to induce the directed differentiation of BMSCs to chondrocytes, and to explore the best inductive effect. METHODS: Rat BMSCs were isolated, cultured and purified using adherent culture. Then, different inducing factors were added in the induction medium: TGF-β1+IGF-1 group, TGF-β1 group, IGF-1 group, and control group without growth factors. Immunofluorescence was carried out at 21 days of induction. The expression of collagen type Ⅱ was evaluated by immuncytochemical staining at 7, 14 and 21 days of induction. RESULTS AND CONCLUSION: (1) Immunofluorescence detection of the TGF-β1+IGF-1 and TGF-β1 groups showed highly expressed collagen type Ⅱ (brown red-stained cytoplasm), while negatively expressed collagen type Ⅱ in the other two groups. (2) Findings from the immuncytochemical staining showed that the expression of collagen type Ⅱ was stronger in the TGF-β1+IGF-1 group than the TGF-β1 group (P < 0.01), and the expression was gradually enhanced with time. Meanwhile, there was also no expression of collagen type Ⅱ in the IGF-1 and control groups. To conclude, the combination of TGF-β1 and IGF-1 can achieve the better inductive effect on the chondrogenic differentiation of BMSCs in vitro.关键词
软骨细胞/诱导分化/胰岛素样生长因子1/转化生长因子β1/干细胞/骨髓间充质干细胞分类
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谭荣邦,陈宏明,罗世官,李日著,曾德创..体外诱导骨髓间充质干细胞定向分化为软骨细胞:转化生长因子β1、胰岛素样生长因子1的协同刺激作用[J].中国组织工程研究,2018,22(17):2631-2636,6.
